【作者】 梁瑜祯；

【导师】 夏宁； 肖常青； 王乃尊；

【作者基本信息】 广西医科大学 ， 内分泌， 2003， 硕士

【摘要】 目的 1、 研究广西壮、汉族人群载脂蛋白CIII（ApoCIII）基因SstI位点多态性，以获得广西壮、汉族人群ApoCIII基因频率分布资料;同时与西方白种人相比，探讨ApoCIII基因SstI位点的人种差异。2、探讨ApoCIII基因SstI酶切位点多态性与2型糖尿病及高脂血症的关系。方法 采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测114例汉族人和107例壮族人及122例2型糖尿病患者的ApoCIII基因SstI位点多态性。结果 ① 广西壮、汉族人群ApoCIII基因SstI位点S1等位基因的频率分别为0.706、0.772，S2等位基因频率分别为0.294、0.228，壮、汉族比较,壮族人群S2等位基因频率有上升的趋势，无显著性差异（P＞0.05）；西方白种人S1及S2等位基因的频率为别为0.879、0.012,与广西壮、汉族人群相比有显著性差异（P＜0.05＝。② 2型糖尿病患者与对照组ApoCIII基因SstI位点在两组中均以S1等位基因为主，两组比较S2等位基因频率无显著性差异（0.262 vs 0.228，P＞0.05）,高甘油三酯组（HTG）S2等位基因频率与正常甘油三酯（NTG）组无显著性差异（2型糖尿病组：NTG vs HTG:0.299 vs 0.312,对照组：NTG vs HTG：0.203 vs 0.283）；③ApoCIII基因SstI基因型与体重指数、收<WP=4>缩压、舒张压、胆固醇、甘油三酯、低密度脂蛋白和载脂蛋白A1、载脂蛋白B和HOMA胰岛素抵抗指数相比无显著性差异（P＞0.05）。结论 ①广西壮、汉族人群与白种人相比ApoCIII基因SstI酶切S2等位基因位点有显著性差异，存在明显的人种差异；壮族人群S2等位基因位点有上升的趋势，但无显著性差异，说明广西壮、汉族人群有同源性。② ApoCIII基因SstI酶切位点多态性与2型糖尿病及HTG无关。更多还原

【Abstract】 Objective: 1. To investigate the apolipoprotein CIII(ApoCIII) SstI polymorphism in Guangxi Zhuangzu and Hanzu people.2. To study the apolipoprotein CIII(ApoCIII) SstI polymorphism and its relationship with the type 2 diabetic patients and the hypertriglyceridemia pepole. Methods: The genotype and allele fragment of ApoCIII gene SstI polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）in 114 Hanzu people,107 Zhuangzu people and 122 patient of type 2 diabetes in Guangxi. Results: 1、The frequencies of S1 alleles and S2 alleles were 0.706 , 0.772 and 0.294,0.228 in the Zhuangzu and Hanzu people in Guangxi respectively.The frequencies of S2 alleles were not significantly difference between the Zhuangzu and Hanzu people in Guangxi （p＞0.05）.The frequencies of S1 and S2 alleles in the Westen white men were 0.897 and 0.012 respectively. There were significantly difference between the Westen white men and the Zhuangzu and Hanzu people （p＜0.001 and p＜0.05).2、In the type<WP=6>2 diabetic group and the control group , S1 alleles were the major alleles,homozygous S1S1 genotype were the frequent one in both groups. There were not significantly diffrence in the frequence of S2 alleles between the type 2 diabetes and the control groups.No significantly differences were found in ApoCIII gene SstI polymophism between the high triglyceride(HTG) subjects and normal triglyceride(NTG) subjects .3、There were not significantly difference in the body index，systolic pressure, diastolic pressure,cholesterol, triglyceride, low density lipoprotein,ApoA1,ApoB, ApoA1/B ,HOMA-Insulin resistance index among the S1S1, S1S2 and S2S2 genotypes（p＞0.05）.Conclusion:The ApoCIII gene polymorphism were not significantly defference between the Zhuangzu and Hanzu people in Guangxi, but there were not significantly defference between the Guangxi people and West white men. No significantly differences were found in ApoCIII gene SstI polymophism between type 2 diabetic group and control group, and the ApoCIII gene SstI polymophism was not associated with hypertriglyceridemica.更多还原