【作者】 郭颀然；

【导师】 吴兆亮；

【作者基本信息】 河北工业大学 ， 化学工艺， 2003， 硕士

【摘要】 D-核糖是一种重要的生理物质，常用于食品工业和医疗工业中，如调味品的生产和维生素B₂的合成，近几年来，随着发酵生产D-核糖研究的不断深入，发酵液中D-核糖的分离纯化过程已成为重要的研究课题。本文以D-核糖发酵液为研究体系，对发酵液中D-核糖的分离纯化过程进行了研究。 本论文利用活性炭对发酵液脱色；利用阴阳离子交换树脂脱盐；聚砜型超滤膜超滤除蛋白质；最后结晶得到D-核糖。主要内容如下： 1．研究了D-核糖发酵液活性炭脱色工艺和数学表征，为其工艺的优化设计和操作提供依据。实验表明，用活性碳脱色在间歇操作条件下最佳的吸附时间为30 min，操作温度为50℃，pH为3.5。建立色素吸附等温线和计算式，其等温线的类型为L型。 2．研究了有机溶剂对D-核糖发酵液活性炭脱色的影响，建立了对应的吸附等温线Q=1.870C²／（1+48.5C²），确立了其操作工艺，其最佳的吸附时间为30min，操作温度为50℃，pH为3.5。 3．利用201×7强碱型阴离子交换树脂和001×4强酸型阳离子交换树脂去除D-核糖发酵液中的阴阳离子，对D-核糖发酵液离子交换树脂脱盐工艺进行了研究。实验表明，采用2.5倍发酵液体积的去离子水对脱盐后的树脂进行清洗可得到最大的D-核糖收率，溶液电导率可减小到759μs／cm，盐度减到0.5 ppt，核糖收率达到80％以上。 4．在研究发酵液离子交换树脂脱色吸附等温线和工艺的基础上，结合活性炭吸附工艺，建立了发酵液活性炭和离子交换树脂联合脱色的工艺模型，为降低分离成本提供了理论和技术。 5．利用膜过滤脱除发酵液中的蛋白质省能、高效，并且考察了膜表面特性对蛋白质脱除程度的影响。实验表明膜分离操作对D-核糖发酵液中蛋白质能起到很好的截留作用。调整发酵液pH值为5.5-6.5，加入15％的乙醇能使蛋白质截留率达到90％。 6．对反应结晶法分离D-核糖进行了初步研究，并与浓缩结晶法进行了比较，提出了进一步研究的方向。更多还原

【Abstract】 D-ribose, which is important for physiology, can be made into VB2, flavor agent and various nucleic acid medical. In the recent years, with the development of D-ribose fermentation, the process of separation and purification of D-ribose had been very important aspect in research. On the basis of the ferment system of microbe, this paper studied the the process of separation and purification of D-ribose in the fermentation broth.In this paper, fermentation discolored by active carbon firstly; and secondly, desalinated by ion-exchange resin; removed proteins by polysulfone ultrafiltration membrane thirdly; finally, acquired D-ribose by crystal. The object matter as follows:l,The technology and expression of adsorbent decolorization of fermented broth for production of D-ribose was studied, which was measured easily and correctly and expounded the computation methods on technology of the adsorbent decolorization of fermented broth. The optimum technologies of the adsorbent decolorization of fermented broth by active carbon were adsorbent time 30 min, temperature 50 C and pH 3.5. Adsorption isotherm and the corresponding equation were obtained, of which kind was type-L.2,The influential factors of active carbon discoloration influenced by organic solvent and the corresponding changing discipline had been studied. The corresponding adsorption isotherm, Q=1870C2/(1+48.5C2), had been established. The optimum conditions were chosen as follows: pH=3.5, T=40-50 C, and stirring time: 30min.3, Removed anions and cations by 201+7 anion exchange resin and 001 x4 cation exchange resin, and the desalination technology in D-ribose fermented broth was studied.The maximal recovery ratio of D-ribose can be get after rinsing desalinated resin by using deionized water that is 2.5 times ferment broth volume, conductance radio can be decreased to 759us/cm, salinity 0.5 ppt,and the recovery ratio of D-ribose achieved 80% above.4, The adsorption isotherm of ion-exchange resin discoloration was conformed and the unit model which active carbon combine with ion-exchange resin discoloration was made .By computing this model, the optimal process conditions were established, and the theories and technic for reducing separating charge in the industrialized production were provided.5, Removed proteins in the fermented broth by polysulfone ultrafiltration membrane were studied, and the effects of membrane surface characteristic on the degree of proteins deprivation was studied.It was a good operation that entrap the proteins of D-ribose ferment broth by using membrane separation. When the ferment broth pH 5.5-6.5 and ethanol concentration 15%,the protein entrapment ratio achieved 90%.6, Separated D-ribose by the reactive crystallizing was studied preliminary, and the condensing crystallizing for extract D-ribose was compared, and put forward the study direction in the future.更多还原