【作者】 臧运华；

【导师】 李震；

【作者基本信息】 山东中医药大学 ， 中西医结合基础， 2003， 硕士

【摘要】 目的：探讨“益气养阴方”治疗白血病的作用机理。方法：运用血清药理学方法孵育人白血病细胞系K562细胞，通过荧光光度测定法、流式细胞术、免疫组化法分别检测灌胃大、中、小剂量的“益气养阴方”大鼠的血清对膜流动性、细胞周期和细胞凋亡及bcl-2、突变型p53基因表达的影响。结果：小剂量组对细胞膜流动性无明显影响。而大剂量组使K562细胞膜流动性增高，表现出与注射砒霜大鼠的血清和灌胃羟基脲大鼠的血清相同的趋势；灌胃三种剂量“益气养阴方”大鼠的血清均能使K562细胞S期细胞百分数增加，GO-G1期细胞百分数减少，与注射砒霜大鼠的血清影响结果相似。三种剂量的血清均能使K562细胞bcl－2和突变型p53基因表达减少，它们都能诱导K562细胞调亡。结论：灌胃大、中、小剂量“益气养阴方”大鼠的血清均能使K562细胞阻滞于S期，并且诱导K562细胞调亡。这种作用可能与其使K562细胞膜流动性增高及bcl－2和突变型p53基因表达减少有关。更多还原

【Abstract】 Objective To explore the mechamism of treating leukemia with Yiqi Yangyin formula. Methods We hatched cell lines K562 with human leukemia by means of serological pharmacology. At the same time, Yiqi Yangyin formula is given to the rats. And then, we observe rats’ serum’s effects on membrane fluidity, cell cycle and bcl-2 gene and mutant p53 gene expression of cell lines K562 by fluorophotometry, flow cytometry and immunohistochemic. Results Serum of the rats given Yiqi Yangyin formula at lower drug concentrations has little effect on the membrane fluidity. But at higher drug concentrations it can increase membrane fluidity of cell lines K562, this effect has the same tendency to serum of the rats given arsenical agents and that given HU. Serum of the rats given Yiqi Yangyin formula can increase membrane fluidity and S phase cells’ percentage of cell lines K.562 and decrease GO-G1 phase cells’ percentage, which is similar to the effect of the rats’ serum that are given arsenical agents. Serum of the rats given Yiqi Yangyin formula down-regulates the expression of bcl-2 gene and mutant p53 gene, and in addition it induces the apoptosis of the cell lines K562. Conclusion With serum of the rats given Yiqi Yangyin formula, S phase cells of cell lines K562 increased, and then apoptotic cells increased with a diminution of S phase cells. This mechanism may relate to the function that increase membrane fluidity of cell lines K562 and down-regulate the expression of bcl-2 and mutant p53 gene.更多还原