【作者】 邓献存；

【导师】 郑连英；

【作者基本信息】 浙江大学 ， 生物化学工程， 2003， 硕士

【摘要】 筛选出能以甲苯为唯一碳源的菌株，并以筛选出的野生有降解甲苯能力的菌株作为诱变出发菌，对其进行了紫外光和LiC1的复合诱变，得到一株降解甲苯能力提高4.8％的诱变突变株，经初步鉴定为假单孢菌，命名为Pseudomonas sp．ZD5A。 设计出甲苯废气净化装置—生物过滤器，并对梧桐树枝、杉树皮、陶瓷环、不锈钢拉西环四种生物过滤器填料的性能进行了评选，梧桐树枝为生物过滤器最优填料。在φ5×13cm有机玻璃生物过滤器内，当温度为30℃，入口气体流量为0.01m~3／h，填料的相对湿度为80％，培养基pH值为7.0，甲苯废气浓度小700mg／m~3时，以梧桐树枝为填料的生物过滤器净化效率在90％以上。 对填充有空隙率为0.57的梧桐木块填料，填料有效高度分别为25cm和67cm的生物过滤器(φ15cm)处理甲苯废气的操作条件进行了评价。φ15×25cm的生物过滤器处理条件为：温度为30℃，入口气体流量小于0.3m~3／h，填料的相对湿度为80％，培养基pH值为7.0，在入口甲苯废气浓度小于3000mg／m~3的条件下，生物过滤器的甲苯废气净化效率在90％以上。φ15×67cm的生物过滤器处理条件为：温度为30℃，入口气体流量小于0.5 m~3／h，填料的相对湿度在80％，培养基pH值为7.0，在入口甲苯废气浓度小于5000mg／m~3的条件下，生物过滤器的甲苯废气净化效率在87％以上。 在研究生物过滤器净化低浓度甲苯废气机理的基础上，依据吸附-生物膜理论，选择了生物过滤器降解甲苯废气的动力学模型。并应用该模型计算公式对入口气体甲苯浓度、气体流量、及生物填料层高度等主要因素的影响进行了模拟研究，模拟计算值与实验值之间有较好的相关性。更多还原

【Abstract】 The strain which had the ability to utilize toluene as a sole carbon source was screened and then induced by UV radiation and LiCl, The mutant which has 4.8% higher toluene-degradation efficiency was further identified as Pseudomonas sp. ZD5A.A laboratory biofilter was designed to purify low-concentration gaseous toluene and VOCs in which the Pseudomonas sp. ZD5A was inoculated on its medium. Four kinds of medium(wood block medium, cortex medium,ceramic loop medium and stainless steel loop) were used in the experimental program and the wood block medium was found to be the best one.For the biofilter(φ5 x 13cm) with wood block medium,toluene removal efficiency at an inlet concentration of less than 700mg/m3 was greater than 90% under the conditions that the temperature was 30℃,the inlet flux was 0.01m3/h, relative moisture of the medium was 80% and pH=7.0.The operating parameters were optimized for two biofilters(φ15x25cm and φ 15x67cm) filled with wood block medium of which porosity was 0.57. For the biofilter(φ15x25cm),when the temperature was 30℃,the inlet flux was less than 0.3m3/h, relative moisture of the medium was 80% and pH=7.0, the toluene removal efficiency at an inlet concentration of less than 3000mg/m3 was greater than 90%. For the biofilter(φ15x 67cm),when the temperature was 30℃,the inlet flux was less than 0.5m3/h, relative moisture of the medium was 80% and pH=7.0, toluene removal efficiency at an inlet concentration of less than 5000mg/m3 was greater than 87%.Based on the process of purifying gaseous low-concentration toluene,the biodegradation mechanism and the new Adsorption-Biofilm theory,a Kinetic model was set up.In further simulation study for the main factors,such as the inlet toluene concentration,the gas flux and the height of biofilm-packing,a good pertinence was showed between calculated data and test data.更多还原