【作者】 方静；

【导师】 崔恒敏；

【作者基本信息】 四川农业大学 ， 基础兽医学， 2002， 硕士

【摘要】 通过建立雏鸭锌缺乏（锌含量：22.90mg／kg）锌中毒（锌含量：1300mg／kg）的病理模型，研究锌对免疫器官生长发育及免疫功能的影响。缺锌和锌中毒组免疫器官的绝对重量、生长指数、细胞生长周期以及T淋巴细胞ANAE⁺阳性率、红细胞C₃b受体花环、免疫复合物花环率与对照组比较差异显著（p＜0.05）或极显著（p＜0.01）。病理组织学观察免疫器官淋巴细胞显著减少并呈退行变，网状细胞变性坏死。超微结构显示缺锌组淋巴细胞核固缩或溶解，线粒体、粗面内质网受损；胸腺网状细胞核受损，粗面内质网扩张，线粒体肿胀、嵴断裂。锌中毒组淋巴细胞线粒体受损，网状细胞出现大量各级溶酶体、吞噬体。结果表明，缺锌和锌中毒严重抑制雏鸭免疫器官生长发育和外周血T淋巴细胞活化，并对免疫器官造成明显的病理损伤，导致细胞、体液、红细胞免疫功能下降。更多还原

【Abstract】 By establishing the phatholoical model of zinc deficiency (zinc content: 22.90mg/kg) and toxicity (zinc content:1300mg/kg) in ducklings , the effects of zinc deficiency and toxicity on the development of immune organs and immune functions were studied. Campaired with the control groups ,the absolute weight ,grouth index and cell cycle of immune organs, the ANAE positive rates of T lymphocytes in peripheral blood , the erythrocyte C3bRR and CIC rosette rates were singnificantly changed in the zinc-deficient and zinc-toxic ducklings. Histopathologically, lymphocytes of the immune organs were depleted,degenerated and necrotic in both zinc-deficient and zinc-toxic ducklings. Ultrastructurally,karyopyknosis and karyolysis occured in lymphocytes,and the mitochondria ,rough endoplasmic reticulum of lymphocytes in the immune organs were damaged .The nucleus were deformity ,the rmtochondrial and rough endoplasmic reticulum were swelling ,and the ribosomes were shed in reticular cells of thymus in zinc-deficient ducklings. The mitochondrial in lymphocytes of the immune organs were damaged, and reticular cells consisted of many primary and secondary lysosoms as well as phagocytes in zinc-toxic ducklings. Results demonstrated that zinc deficiency and toxicity greatly suppressed the development of immune organs, caused obvious pathological injury of the immune organs, weakened the funcion of humoral,cellular and erythrocyte immunity.更多还原