【作者】 耿霞；

【导师】 梁冰；

【作者基本信息】 四川大学 ， 应用化学， 2002， 硕士

【摘要】 对恶性肿瘤化疗减毒增效复方中药中的化学成分进行研究，分离纯化其有效成分，对促进我国中医中药的开发和中医中药治疗肿瘤的医疗水平的提高有很大的社会意义。本文采用中药化学成分系统预试的方法对恶性肿瘤化疗减毒增效复方中药口服液中所含化学成分进行了较全面的鉴定，认为该复方中存在氨基酸类、内酯、香豆精及其甙类、生物碱类、皂甙类、糖、多糖及其甙类、鞣质及酚类、强心甙等成分。采用多级乙醇沉淀将上述复方中在75％～25％乙醇浓度范围内不溶的多糖组分进行了初步分离，透析除去小分子杂质，经DEAE-纤维素柱层析，依次用水、0.5mol/L NaHCO₃溶液、1mol/L NaHCO₃溶液、0.5mol/L NaOH溶液洗脱，得多糖组分P、P₁、P₂、P₃；其中P₁，再经Sephadex G-150凝胶柱层析分离得一水溶性多糖组分HMP-2。经HPLC和IR分析，证实P₁是纯度为97％的多糖类物质，且具有β-呋喃糖醛酸甙的结构。P经HPLC分析表明为两个分子量相当接近的多糖组分的混合物，再经SephadexG-150柱层析，无法再将其进一步分离。 本文研究还发现多糖—苯酚—硫酸显色反应体系的一阶导数光谱值与多糖含量成正比，据此建立了苯酚—硫酸显色导数光谱分析多糖的方法。它具有选择性好、不受样品颜色的干扰、所需样品量少、简便、快速、准确的优点。直接应用于甘草、黄芪、当归、女贞子、红参、枸杞、茯苓等单味药水提液及恶性肿瘤化疗减毒增效复方中药口服液中多糖含量的测定，取得了满意的结果。更多还原

【Abstract】 This thesis investigate the isolation and determination of polysaccharides in a Chinese herbs complex prescription used in clinical study for malignant tumor chemotherapy by Chengdu university of traditional Chinese medicine, claimed to have outstanding effect to attenuate adverse effect and enhance curative effect.First, the Chinese herbs complex prescription (CHCP) oral drug was systematically pretested. It was found that there were amino acid, lactone, coumarin, coumarin glycoside, alkaloid, saponin, saccharide, polysaccharide, tannin, phenolic and cardiac glycoside in it. Crude polysaccharides in the CHCP oral drug were isolated preliminarily by multiple deposition in ethanol of 75%~25%. Micro molecule components in crude polysaccharides were removed by dialyze. Four fractions of Polysaccharides (P,P1,P2,P3) qualitatively determined by phenol- sulfuric acid method were eluted out in turn from DEAE- cellulose column chromatography with H2O, 0.5mol/L NaHCO3, 1 mol/L NaHCO3 and 0.5mol/L NaOH. A polysaccharide, HMP-2, was isolated from P1 by Sephadex G-150 column chromatography, with a purity of 97% by HPLC and the structure of ?-furanose uronic acid glycoside identified by IR. P was a mixture of two polysaccharides, which have proximate molecular weight by HPLC and cannot be separated by SephadexG-150 column chromatography.It was also found that first derivative spectrometric value of polysaccharide -phenol- sulfuric acid reaction system is directly proportional to the content of polysaccharides. Basing this, a phenol-sulfuric acid derivative spectrometry for determination of polysaccharides in Chinese herbal medicine is proposed and proved to be an easy, rapid and accurate method with good selectivity, small amount of sample required and little interference of sample matrix color. The method is applied to determination of the content of polysaccharides in the CHCP oral drug (1) and single herbs such as Glycyrrhiza, Astragalus, Angelica etc. with satisfied results.更多还原