【作者】 刘霞；

【导师】 苏云明；

【作者基本信息】 黑龙江中医药大学 ， 中西医结合基础， 2002， 硕士

【摘要】 目的：研究蝙蝠葛酚性碱(PAMD)对大鼠实验性脑缺血的保护作用，并初步探讨其作用机理。方法：采用双侧颈总动脉结扎法制备大鼠不完全脑缺血模型，观察缺血 45min再灌注2h PAMD对脑组织含水量、SOD活性、MDA及NO含量的影响，并观察了PAMD对脑缺血大鼠血栓形成的影响；线栓法制备大鼠大脑中动脉阻塞模型，缺血2h再灌注24h后观察PAMD对脑缺血后细胞凋亡及相关基因bcl-2和bax表达的影响，Tunel法测细胞凋亡，免疫组化法观察bcl-2和bax的表达。结果：PAMD可减少缺血脑组织含水量，减少丙二醛(MDA)和一氧化氮(NO)的产生，增强超氧化物歧化酶(SOD)活性，抑制脑缺血大鼠血栓形成；减少脑缺血引起的细胞凋亡数，并能诱导bcl-2的表达、抑制bax的表达。结论：PAMD对脑缺血有保护作用，其机理可能与抑制血栓形成、抗自由基生成、减少NO的生成和抑制细胞凋亡及影响相关基因的表达有关。更多还原

【Abstract】 Objective: It was studied the protective effect of phenolic alkaloids from Menispermum dauricum(PAMD) on experimental cerebral ischemia in rats and explored the mechanism of action. Methods: The imcomplete cerebral ischemia was induced by the occlusion of bilateral common carotid arteries(CCA), 45min after ischemia ,the occlusion was removed and the blood was rapidlly reinfused. Water content, superoxide dismutase (SOD) activity, malondialdehyde(MDA) and nitric oxide(NO) content of brain tissue were determined and observed the effect of PAMD on thrombosis formation;The focal cerebral ischemia model was produced by occluding middle cerebral artery(MCA) for 2h and reperfusing 24h.Cell apoptosis was detected by Tunel,the expression of bcl-2 and bax were analyzed by immunohistochemical.ResuIts:PAMD can decrease water content of brain tissue,reduce producion of MDA and NO,enhance activity of SOD and inhibit thrombosis formation;reduce cell apoptosis after ischemia reperfusion,induce bcl-2 expression and inhibit bax expression.Conclusion:PAMD processes protective effect on experimental cerebral ischemia.The mechanism of action is relate to reduce production of free radical and NO,anti-thrombosis formation and inhibit cell apoptosis.更多还原