【作者】 刘树英；

【导师】 李亚东；

【作者基本信息】 吉林农业大学 ， 果树学， 2002， 硕士

【摘要】 以兔眼越桔(Vaccinium ashei Reade)的粉蓝、精华、杰兔、巨丰、芭尔德温、蓝美人和园蓝等7个品种的二年生树上的幼嫩茎段为试材，进行芽诱导、增殖及生根试验。实验结果表明：(1)MS、WPM、Knop、Anderson培养基都适宜于兔眼越桔芽诱导增殖，MS和WPM培养基较Knop和Anderson培养基表现好，与其它类群比较发现，基因型对越桔的组织培养具有很大的影响；(2)在芽诱导和继代过程中添加NAA，2ip，有利于兔眼越桔芽的增生，而且2ip对再生能力具有保持作用，激素组合以2ip 15mg／L+NAA0.02mg／L为好；(3)在继代培养中对光照强度，pH值，温度做了实验。发现光照强度为3000Lux，4000Lux处理时效果较好；pH值为5.4时较6.8时好；温度为30℃时较20℃和40℃好；(4)在生根培养基中添加适量的IBA，NAA可有效促进生根，但以IBA效果好。(5)瓶外生根用速蘸IBA法，结果发现浓度为1500，2000，2500mg╱L时效果较好，其效果明显优于瓶内生根，生根率可达80％以上。更多还原

【Abstract】 Using Powder , Choice,Primer, Dellite, Balduin, Bluebelle, Gardenblue as plant materials and the micropropagation procedure of rabbiteye b\uebeny(Vaccinium ashei Reade) and several factors were studied . It was discovered that MS and WPM are more effective than Anderson and Knop and good medium were established successfully. The results also indicated: supplementing the induction and subculture medium with NAA, 2ip benefited the induction of blueberry buds, but 2ip maintained the ability of differentiation. Plant source especially genotype played no role on the bud differentiation of long-term culture; Different hormones (NAA,2ip and ZT etc.) affected the buds differentiation frequency. Among them, 2ip increased the frequency of bud formation, increased the frequency of the bud differentiation frequency; In subculture stage, we found that 30001ux, 40001ux light bud formation. In autumn, the explant which was pre-handled by low-temperature could enhance intensity was better than others; supplementing the root medium with IBA or NAA increased the formation of root, but IBA was the better one.更多还原