【作者】 汪乐余；

【导师】 王伦；

【作者基本信息】 安徽师范大学 ， 分析化学， 2002， 硕士

【摘要】 鉴于半导体纳米粒子优良的光学特性，如：激发光谱宽，发射光谱窄而对称，光化学稳定，荧光寿命长，荧光量子产率高等，将其应用于生物大分子的分析测试，势必提高分析的灵敏度和选择性。 本文合成了无机半导体纳米粒子CdS和ZnS，并以巯基乙酸对CdS纳米粒子进行了功能化修饰，以巯基乙酸钠对ZnS纳米粒子进行了功能化修饰。改善了纳米粒子的水溶性，并使其能同生物大分子发生作用。以红外光谱、紫外-可见吸收光谱、荧光光谱和透射电子显微镜对纳米粒子进行了表征和性质研究。最终将巯基乙酸修饰的CdS纳米粒子成功地用于蛋白质和核酸的定量分析。以荧光增敏法测定了人血清蛋白质，对人血清白蛋白测定时，线性范围为0.2～3.5μg mL^-1，检出限为0.09μg mL^-1，线性相关系数为0.9991。对人血清中γ-球蛋白测定的线性范围为0.2～11.0μg mL^-1，检出限为0.18μg mL^-1，线性相关系数为0.9979。以荧光猝灭法测定了小牛胸腺DNA，线性范围为0.1～1.5μg mL^-1，检出限为0.018μg mL^-1，线性相关系数为0.9989。方法简单、快速、灵敏。更多还原

【Abstract】 Semiconductor nanoparticles have a narrow, tunable, symmetric emission spectrum and a broad, continuous excitation spectrum. They are also photo-chemically stable and they have high fluorescence quantum yields and long fluorescence lifetime. The high selectivity and sensitivity should be acquired when the nanoparticles are used to detect the macromolecules.In this work, nano-ZnS and nano-CdS have been successfully prepared. The nano-CdS have been modified with mercaptoacetic acid, and nano-ZnS have been modified with sodium mercaptoacetic acid. The functionalized nanoparticles are water soluble ,and biocompatible. All the nanoparticles have been characterized by IR spectra, UV spectra, Fluorescence spectra and TEM images. In comparison with single organic fluorophore, the nanoparticles probes are brighter, more photostable, and do not suffer from blinking. The functionalized nano-CdS has been used as fluorescence probe in determination of Human Serum protein. Under the optimal conditions, the response is linearly proportional to HSA concentration between 0.2ug mL-1 and 3.5/ig mL-1, and the limit of detection is 0.09ug mL-1. The response is linearly proportional to Y -G concentration between 0.2ng mL-1 and 11 .Oug mL-1, and the limit of detection is 0.18ag mL-1. A fluorescence method has also been developed for rapid determination of ct-DNA with functionalized CdS as a fluorescence probe, based on the fluorescence quenching of functionalized CdS in the presence of ct-DNA. Under optimal conditions, the calibration graph is linear over the range 0.1~1.5jig mL-1for calf thymus DNA (ct-DNA). The corresponding detection limit is 0.018g mL-1, and the linear correlation coefficient is 0.9989. The assay was proved to be a simple, rapid and specific method. The recovery and relative standard deviation are very satisfactory.更多还原