【作者】 高峰；

【导师】 王伦；

【作者基本信息】 安徽师范大学 ， 分析化学， 2002， 硕士

【摘要】 蛋白质和DNA都是重要的生物大分子。对蛋白质和DNA进行定量测定是生物化学和生命科学中经常涉及的分析内容，建立高灵敏度、低检测限的荧光探针定量测定的方法仍具有重要意义。 本论文以阳离子染料灿烂甲酚蓝在阴离子表面活性剂十二烷基磺酸钠存在下形成的现场弱荧光二聚体为荧光探针，建立了测定牛血清白蛋白的新方法，该法线性范围为0～7mg/L，检测限为3.66×10^-3mg/L，对2.00 mg/L几的牛血清白蛋白平行测定8次，相对标准偏差为2.77％，说明本法准确可靠。以阳离子染料耐尔蓝在阴离子表面活性剂十二烷基苯磺酸钠存在下形成的现场弱荧光二聚体作为测定人血清总蛋白的荧光探针。该法用于实际样品的测定，与临床方法测定的结果相吻合。 染料二聚体也是能量转移的有效形式。本论文以吖啶橙—罗丹明B二聚体能量转移体系作为荧光探针，建立了测定DNA的新方法。用于鲱鱼精DNA和小牛胸腺DNA的测定，线性范围分别为0.33～1.33mg/L，0.33～3.33mg/L；检测限分别为1.63×10^-3mg/L，1.52×10^-3mg/L。对1.00mg/L鲱鱼精DNA和小牛胸腺DNA的测定，相对标准偏差分别为2.40％和2.00％。更多还原

【Abstract】 Protein and DNA are important biology macromolecules. The quantitative determination of protein and DNA is a common analysis contents in biology chemistry and life science. So it is very important to find fluorescence probe with high sensitivity and low detection limit. In this thesis the in situ brilliant cresyl blue (BCB) dimer with weak fluorescence in the solution of anionic surfactant (AS) sodium lauryl sulfonate (SLS) was used as fluorescence probe. At the same time, thenew method for the determination of bovine serum albumin(BSA) was built. Thelinear range was 0-7mg/L and the detection limit(3 o /k)was 3.66 10-3 mg/L,theR.S.D for the determination of 2.00mg/L BSA was 2.77%. So the method was accurate and reliable.The in situ nile blue (NB) dimer with weak fluorescence in the solution of anionic surfactant sodium dodecylbenzene sulfonate-6(DBSS) was also used as the fluorescence probe for the determination of total proteins in human serum. The determination results achieved by this method were in good agreement with clinical data provided by the hospital when this method was used to determinate total proteins in real human serum samples.Dye dimer is effective form of energy transfer, too. In this thesis, the energy transfer system of acridine orange(AO)-rhodamine B(RB) dimer was built for the determination of DNA as fluorescence probe. Under the experimental conditions, the linear tange of this assay was 0.33~1.33mg/L for fish sperm DNA and 0.33-3.33mg/L for calf thymus DNA,the detection limit was 1.63xlO"3mg/L for fish sperm DNA and 1.52x10-3 mg/L for calf thymus DNA,respectiveIy.The R.S.D for the determination of 1.00mg/L DNA was 2.40% for fsDNA and 2.00% for ctDNA,respectively.更多还原