【作者】 周国利；

【导师】 金海国； 储明星；

【作者基本信息】 延边大学 ， 动物遗传育种与繁殖， 2002， 硕士

【摘要】 本研究分析了7个微卫星座位BM1818、BM1258、 BM1443、BM1905、BM302、BM4505和CYP21在240头奶牛群体中的遗传变异（PIC、遗传杂合度、有效等位基因数和基因频率）。结果显示7个微卫星在群体中的PIC、遗传杂合度和有效等位基因数的范围分别为0.3869～0.7144、0.4348～0.7508和1.7693～4.0124。利用最小二乘法拟合线性模型对不同标记基因型间体细胞数差异显著性检验结果表明：微卫星BM1818座位基因型为284bp/284bp所对应的体细胞数最小二乘平均值显著低于286bp/286bp所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因284bp与体细胞数呈显著正相关，286bp与体细胞数呈显著负相关；微卫星BM1258座位基因型为100bp/100bp所对应的体细胞数最小二乘平均值明显低于102bp/102bp、106bp/106bp、106 bp / 104 bp 、106 bp/102bp、106bp/100bp和104bp/100bp所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因100bp与体细胞数呈显著的正相关，102bp、104bp和106bp与体细胞数呈显著负相关；微卫星BM1443座位基因型为166bp/160bp和166bp/166bp所对应的体细胞数最小二乘平均值明显低于170bp/160bp、160bp/157bp和165bp/160bp所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因166bp与体细胞数呈显著正相关，157bp、165bp和170bp与体细胞数呈显著负相关；微卫星BM1905座位基因型为187bp/187bp所对应的体细胞数最小二乘平均值明显低于197bp/195bp和193bp/187bp所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因187bp与体细胞数呈显著正相关，193bp与体细胞数呈显著负相关；微卫星BM302座位基因型为142bp/140bp所对应的体细胞数最小二乘平均值明显低于154bp/145bp和143bp/143bp所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因142bp和140bp与体细胞数呈显著正相关，143bp、145bp与体细胞数有明显负相关；微卫星BM4505座位基因型为240bp/236bp所对应的体细胞数最小二乘平均值明显低于240bp/238bp、<WP=5>240bp/239bp、240bp/219bp、236bp/219bp和235bp/219bp所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因236bp与体细胞数呈显著正相关，239bp、238bp和219bp与体细胞数呈显著负相关；微卫星CYP21座位基因型为215bp/198bp所对应的体细胞数最小二乘平均值明显低于除215bp/194bp以外其它所对应的体细胞数最小二乘平均值（P<0.05），并且等位基因215bp与体细胞数呈显著正相关，208bp、204bp、198bp、194bp和192bp与体细胞数呈显著负相关。更多还原

【Abstract】 The genetic variation (polymorphic information content (PIC), heterozygosity, effective number of alleles and gene frequency) of 7 microsatellite loci (BM1818, BM1258, BM1443, BM1905, BM302, BM4505 and CYP21) was analyzed in 240 cow populations. The results show that PIC, heterozygosity and effective number of alleles of 7 microsatellite loci range from 0.3869 to0.7144，from 0.4348 to 0.7508, from 1.7693 to 4.01249, respectively. Least squares mean of somatic cell counts(SCC) for BM1818 284bp/284bp was significantly lower than those for BM1818 286bp/286bp (P<0.05) in cow populations. The allele 284bp of BM1818 had a significant positive correlation with somatic cell counts in cow populations, the allele 286bp of BM1818 had a significant negative correlation with somatic cell counts in cow populations；Least squares mean of somatic cell counts for BM1258 100bp/100bp was significantly lower than those for BM1258 102bp /102bp, 106bp/106bp, 106bp/104bp, 106bp/102bp, 106bp/100bp, 104bp/100bp (P<0.05) in cow populations. The allele 100bp of BM1258 had a significant positive correlation with somatic cell counts in cow populations, both alleles 102bp, 104bp and 106bp of BM1258 had a significant negative correlation with somatic cell counts in cow populations; Least squares mean of somatic cell counts for BM1443 166bp/160bp and 166bp/166bp was significantly lower than thoseforBM1443 170bp/160bp, 160bp/157bp, 165bp/160bp (P<0.05) in cow populations. The allele 166bp of BM1443 had a significant positive correlation<WP=7>with somatic cell counts in cow populations, both alleles 157bp, 165bp, and 170bp of BM1443 had a significant negative correlation with somatic cell counts in cow populations; Least squares mean of somatic cell counts for BM1905 187bp/187bp was significantly lower than thoseforBM1905 197bp/195bp, 193bp/187bp (P<0.05) in cow populations. The allele 187bp of BM1905 had a significant positive correlation with somatic cell counts in cow populations, the allele 193bp of BM1905 had a significant negative correlation with somatic cell counts in cow populations；Least squares mean of somatic cell counts for BM302 142bp/140bp was significantly lower than those for BM302 154bp/145bp, 143bp/143bp (P<0.05) in cow populations. Both alleles 142bp and 140bp of BM302 had a significant positive correlation with somatic cell counts in cow populations, both alleles 143bp and 145bp of BM302 had a significant negative correlation with somatic cell counts in cow populations; Least squares mean of somatic cell counts for BM4505 240bp/236bp was significantly lower than those for BM4505 240bp/238bp, 240bp/239bp, 240bp/219bp, 236bp/219bp, 235bp/219bp (P<0.05) in cow populations. The allele 236bp of BM4505 had a significant positive correlation with somatic cell counts in cow populations, both alleles 239bp,238bp and 219bp of BM4505 had a significant negative correlation with somatic cell counts in cow populations; Least squares mean of somatic cell counts for CYP21 215bp/198bp was significantly lower than other CYP21 genotypes besids 215bp/194bp (P<0.05) in cow populations. The allele 215bp of CYP21 had a significant positive correlation with somatic cell counts in cow populations, both alleles 208bp, 204bp, 198bp, 194bp and 192bp of BM4505 had a significant negative correlation with somatic cell counts in cow populations.更多还原