【作者】 郝丽梅；

【导师】 魏建昆； 王立安；

【作者基本信息】 河北师范大学 ， 植物学， 2002， 硕士

【摘要】 本研究用电镜技术观察C毒素处理同核的正常细胞质(N)和C型不育细胞质(C)玉米叶片的线粒体，结果表明，C毒素处理后的C细胞质玉米叶片的线粒体外膜完整，内膜受到破坏，嵴破坏严重，有的甚至看不到嵴。而同核的N细胞质玉米叶片的线粒体外膜完整，内膜只受轻微的破坏。未经C毒素处理的同核的N、C细胞质玉米叶片的线粒体内外膜均完整，嵴也很明显。 用氧电极法测定C毒素对同核的N、C细胞质玉米叶片线粒体呼吸速率的影响，结果表明，加C毒素后，C细胞质玉米叶片的线粒体的呼吸速率下降等于或大于20％，呼吸抑制值的差等于或大于0.22；而N细胞质玉米叶片的线粒体的呼吸速率下降等于或小于5％，呼吸抑制值的差等于或小于0.06。 从上述结果可以看出，C毒素对C细胞质玉米线粒体结构和功能的影响具有专化作用，且C毒素的作用位点可能在线粒体内膜上，但是这一推测还有待于在分子水平上的进一步研究。更多还原

【Abstract】 The mitochondria from the normal cytoplasm (N) and CMS-C of Maize treated with C-toxin were observed by a transmission electron microscope (TEM) . The result showed that the outer membrane of C cytoplasmic mitochondria, after treated with C-toxin, appeared intact, the inner mitochondria! membranes were destroyed, and the cristae showed fragmentation to form small vesicles. But the outer membranes of N cytoplasmic mitochondria also appeared intact, and inner membranes were destroyed slightly. The outer and inner membranes of mitochondria not treated with C-toxin all appeared intact, and the cristae showed complete.The respiratory control ratios (RCR) were determined in the presence and absence of C-toxin with a Clark Oxygen electrode. The result showed that RCR declined equal to or more than 0.22 in the presence of C-toxin in C cytoplasm and respiratory ratio declined equal to or more than 20%; but in N cytoplasm RCR declined equal to or less than 0.06 in the presence of C-toxin and respiratory ratio declined equal to or less than 5%.From the above results, it is very clear that C-toxin is specificity to the structure and function of N and C cytoplasmic mitochondria. The site with C cytoplasm exists possible in the membrane of C cytoplasmic mitochondria. But the inference needs to study further in molecular level.更多还原