【作者】 曹庸；

【导师】 饶力群； 罗泽民；

【作者基本信息】 湖南农业大学 ， 生物化学与分子生物学， 2001， 硕士

【摘要】 现代研究表明白藜芦醇（Resveratrol）具有抗癌、抗氧化、抗血小板凝聚、抗菌、调节脂类代谢、保护心血管系统和神经系统等多种功能，从已查文献中可知虎杖中的白藜芦醇含量是迄今研究植物中含量最高的。它具有很高的保健、药用、食用功能和经济价值，故虎杖白藜芦醇的提取、纯化工艺研究正成为人们关注的热点。 1、本论文初步研究了白藜芦醇对光、热的稳定性，研究表明直射的太阳光照射及紫外光照射后白藜芦醇发生变化，但短时间室内放置对白藜芦醇影响较小。白藜芦醇对高温（＞80℃）敏感。长时间室温（120h）放置活性损失大。建立了紫外分光光度法（UV法）、TLC法以及HPLC法分析白藜芦醇的方法。HPLC法采用色谱条件为：色谱柱shim-pack-CLC-OOS，总流速1ml/min，流动相流速比为：A液（重蒸水）：B液（80％乙腈）=0.52ml/min：0.48ml/min，紫外检测波长306nm，检测灵敏度：0.32AT。以HPLC法精度最高，主要可用于最终产品含量测定，而TLC法、UV法均可用于工业生产中的在线检测，且简便、快速，有一定精度。并对虎杖的不同器官，不同季节的样品，以及其它植物中白藜芦醇含量进行了测定，可知含量变化为：根茎的芽＞根茎＞茎＞叶。7月—8月份虎杖白藜芦醇含量最高。在已测定的几种植物中，虎杖的白藜芦醇含量最高。 2、选择3组共28种不同溶剂，研究了这些溶剂对虎杖白藜芦醇提取的影响，从提取液中白藜芦醇提取量及提取液中杂质含量两个方面来看，确定了饱和异丁醇为最佳提取溶剂。其提取效率远远高于目前常用的乙醇碱液提取及丙酮提取，同时有利于微波及超临界CO₂技术的进一步研究及利用。同时进行了不同提取方法研究，确定了采用微波加常规方法进行提取，可以大大提高提取速度，缩短提取时间。 硕士学位论文 虎杖中白膏产醉捉耿、纯化技术研究 曹 偷2（N）Ill 3、研究了从虎杖白基芦醇提取液中分离、提纯白蓉芦醇的工艺条件，通过比较， 表明AB—8，聚酞胺树脂分离、提纯虎杖白基芦醇有较好的效果。通过对国产28种树脂 对虎杖白羹芦醇的静态吸附与解吸性能、动态吸附与解吸性能进行了比较研究，以llPLC 法测定结果为主要依据，从吸附量及解吸量两个方面来看，确定了聚酞胺为最住吸附剂。 解吸试验表明：60％－95％甲醇的溶液可将白葵芦醇为主的成分解吸下来，水及低浓度甲 醇溶液可将大部分杂质解吸除去。然后将60％一95％甲醇解吸液浓缩结晶，可得到白基芦 醇粗品。更多还原

【Abstract】 Modem research shows that resveratrol has multi-functions including the antineoplastic and antioxdant activity. The resveratrol content in polygonun; cuspidatum is the highest among all studied plant species according to known papers. Because it has valuable uses in healthcare, medicine and food , its extraction and purification becomes a research focus.I.. The stability of resveratrol was studied prelimiarly in the paper. The proceduces to analyze resveratrol with UV, TLC and HPLC method were also set up. Different contents were found in different parts of the spmple plant: bud of root> root> stem > leaf Seasonal analysis of the resveratrol in Polygonurn cu.cpidatum indicated the highest content from July to August.2.. 28 kinds of different extract solvents in 3 groups were chosen and the effects of these solvents on resveratrol extraction were studied. It was found that the saturated iso-butand is the best extracting solvent. Meanwhile, through the comparison of different extracting methods, the microwave plus regular methods was reconirnanded.3 . The technological conditions were researched about separating and purifying resveratrol from resveratrol extracts of Polygonum Cuspidaturn. Thecomparison, showed that the effects of AB -8 and polyamide resin were better than others to separate and purify resveratrol of Polygonum cuspidaa’um . Through comparative research of features of 28 kinds of resin of absorption and desorption to the resveratrol of polygonum cuspidwum , polyainide was found to be the best one. The desorption experiment shows that 60%-95% methanol solution can be used to deabsorb the main resveratrol, ~vater and lower32(XiI.iIconcentration of methanol can be used to deabsorb most of tile impurities. And then by concentrating and crystalling with 60%- 95% methanol deabsorbing SQlutIOfl, the coarse resveratrol can be obtained.更多还原