万寿菊根提取物对山楂叶螨几种解毒酶及蛋白质含量的影响

【作者】 王鸿雷；

【导师】 丁起盛； 王金胜；

【作者基本信息】 山西农业大学 ， 生物化学与分子生物学， 2001， 硕士

【摘要】 本文以万寿菊次生物质提取和植物提取物对苹果树山楂叶螨的生物活性测定为基础，采用常规生化实验方法，着重探讨苹果树山楂叶螨在光、暗条件下经万寿菊根氯仿索氏提取物（TPC）作用后几种解毒相关酶类及蛋白质含量的变化规律，生物样品采用活体处理和离体处理相结合的方法，从体内和体外两个角度加以分析。结果表明：山楂叶螨经TPC处理后，谷胱甘肽S-转移酶和蛋白酶活性显著升高，酯酶同工酶受到抑制，蛋白质含量下降，各生化指标变化程度在光照条件下显著高于黑暗处理。更多还原

【Abstract】 Effects of the Extracts of Tagetes Patula on the Activities of Detoxicated Enzymes and Protein Contents in Tetranychus Viennensis Zacher Abstract In this paper, the activities of detoxicated enzyme and protein contents in Tetranychus viennensis Zacher were tested with conventional biochemical method after photo and dark reactivation treated by the chloroform extracts (ThC), which was obtained by sohxlet method from the root of Tagetes patula. The samples were treated in vivo associated with vitro. The results showed that alter the treatment of TPC, the activities of the glutathione S-transferase and protease were remarkably raised, whiles, the esterase isoenzyme were intensively inhibited, and the protein contents declined. The movement degree of the detoxicated enzymes activities of photo treated group is significantly higher than that of dark treated group.更多还原