【作者】 何良军；

【导师】 王根林；

【作者基本信息】 南京农业大学 ， 动物遗传育种与繁殖， 2001， 硕士

【摘要】 本实验建立了MLT间接RIA方法。血液样品先用氯仿萃取MLT后，再用PBS溶解，溶解液用于RIA测定。RIA标准曲线的最小可测浓度是40pg/ml，血液样品的最小可测浓度是15pg/ml，批内误差为9.46％，回收率为85.37％，与6-OH-MLT的交叉率为1.27％。用所建立的RIA方法测定夏至时湖羊和考力代羊全天MLT的浓度，结果表明，考力代羊MLT水平与国外报道结果基本一致，最低浓度大约是20pg/ml，晚间浓度升高，持续时间为9h，峰值是360pg/ml；湖羊最低和最高浓度分别为100pg/ml、1200pg/ml左右，湖羊MLT浓度升高持续时间为17h。说明本实验建立的RIA方法可以用于测定动物血液中MLT的含量。更多还原

【Abstract】 An indirect RIA was developed in order to measure melatomn level in animal blood. Melatonin was abstracted from animal blood with chloroform. The minimum detectable concentration of melatonin with the RLA was 4OpgIml. The minimum melatonin concentration detected in blood was I Spg/ml. The intra-variation was 9.46%. Recovery rate is 85.37% and cross-reactivity of the antiserum with 6-OH-MLT is 1 .27%.Blood samples from Hu and Corridale ewes were measured. Serum MLT concentration in the Corridale measured by the RIA was similar to foreign reports. The nadir of melatonin levels in Corridale ewes was approximately 2OpgIml which lasted about 15 h in day, the duration of elevated levels of 36OpgIml was 9 h. The nadir and peak levels of Flu ewes were 100 and I 200pg/ml, respectively, and the duration of elevated levels was I 7h. The results showed that Hu sheep had higher melatonin level and longer elevated duration than Corridale.更多还原