新混合型卷烟中人参和绞股蓝有效成分分析方法的研究

【作者】 胡震；

【导师】 石进超；

【作者基本信息】 西北大学 ， 物理化学， 2001， 硕士

【摘要】 烟草的化学成分十分复杂，已鉴定出的成分就有4，000多种。卷烟燃吸后，烟气中存在的化学成分在5，000种以上。人参及绞股蓝的有效成分在其中含量很低，本文采用大孔吸附树脂对有效成分进行预分离、富集，在所选定的条件下，回收率在95.7％以上。 卷烟样品分析中对主流烟气的采样选用了国际标准ISO 3308中规定的例行分析用吸烟机，按我国烟草行业标准YC/T29—1996抽吸卷烟，以剑桥滤片捕集主流烟气，用有机溶剂超声提取，分析主流烟气及滤嘴中截留的人参皂苷Rg₁和Re及绞股蓝皂苷含量。 本文首次采用反相高效液相色谱法（RP-HPLC），光电二极管阵列检测器（PDAD），外标法定量检测了新混合型卷烟烟丝、主流烟气及滤嘴中的人参皂苷Rg₁和Re以及绞股蓝皂苷。其中：人参皂苷Rg₁在5.02～20.06μg呈现良好的线性关系，其捕集转移率为18.2％，滤嘴的截留率为16.0％；人参皂苷Re在3.46～34.75μg呈现良好的线性关系，其捕集转移率为6.5％，滤嘴的截留率为11.9％；绞股蓝皂苷在0.11～1.63μg呈现良好的线性关系，其捕集转移率为14.97％，滤嘴的截留率为21.59％。重现性试验中各样品的RSD％在0.23～1.11％范围内。 与文献中常用对一般药物（中草药、中成药等）有效成分分析时所采用的薄层扫描法、分光光度法等相比，本文建立的方法对于新混合型卷烟中中草药有效成分的分析具有专属性好、定量准确、方便实用等特点。 本文的研究课题源自国家烟草专卖局1998年烟草科学技术与技术开发重点项目“新混合型卷烟中中草药有效成分分析方法的研究”（编号：0655），是其中一项重要子课题。 通过本文的研究，建立了一套新混合型卷烟中人参及绞股蓝有效成分的质量控制方法，并从另一角度证明了新混合型卷烟的科学性和有效性；为其它新混合型卷烟的分析和质量标准的制订起到了一定的示范作用；填补了我国新混合型卷烟质量监督工作中的空白，给该类卷烟的研制、生产以科学指导，必将促进我国新混合型卷烟质量控制体系的早日完善。更多还原

【Abstract】 Chemical composition of tobacco is very complicated, though more than 4,000 of components have been identified. After a cigatette is burnt, there are actually more than 5, 000 chemicals in the smoke. The content of the active principles of ginseng and Gynostema pentaphyllum is very low. This research used big梙ole absorption resin to pre梥eparate and collect the active principles. At the given conditions, the recovery rate is 95.7%. In the analysis of the cigarette samples, sample collection of the mainstream smoke was conducted using the regular smoking machine stipulated in ISO 3308. The sample cigarettes were smoked following the standard YC/T29?996 of the tobacco industry of our country. The Cambridge Filter was used to collect the mainstream smoke, and solvents were used to extract through ultrasound to analyze the content of Rg1, Re, and Gypenoside. This research for the first time used reverse high performance liquid chromatography, photoelectric diode array detector and external standard method to determine the contents of Rg1 and Re in ginseng and Gypenoside. It was found that good linear relationship was presented when Rg1 was 5. 02~?0. 06p.g , the collection transfer efficiency was 18.2%, and the filter transfer efficiency was 16.0%. A satisfactory linear relationship was obtained when Re was 3.46?4.75jig , and its collection transfer efficiency was 6.5% and the filter transfer efficiency was 11.9%. Gypenoside showed a good linear relationship when it was 0. IV?. 63~ig. The collection transfer efficiency was 14.97% , and the filter transfer efficiency was 21.59%. Repeatability tests showed the the RSD% in each sample was within 0.23?. 11%. Compared with the normal methods, such as thin條ayer scanning, spectrophotometry, etc., used in analyzing the active principles of normal medicines (medicinal herbs, herbal medicines), the method 3 established by this research is specific, precise in qualitative studies and convenient in analyzing the active principles of the new blend cigarettes. This research came as a branch research topic from a key research project by National Tobacco Monopoly Bureau, which was entitled Studies of the Analyzing Methods in Analyzing the Active Principle oh Herbal Medicines Added to New Blend Cigarettes?(Serial No. 0655). This research is contributive in that it has established a set of methods tha can be used to control and analyze the active principles of ginseng and Gynostema pentaphyllum in new blend cigarettes. It has proved in another angle that the making of new blend cigarettes is scientific and they are effective. In addition, it plays a role as demonstration in the establishment of analysis and quality control methods of other new blend cigarettes. This research has filled a blank of our country in scientific quality control of new blend cigarettes and is significant for guiding scientifically the research and production of the cigarettes of this kind. It will promote early perfection of the national quality control system of new blend cigarettes.更多还原