【作者】 刘海利；

【导师】 吴立军；

【作者基本信息】 沈阳药科大学 ， 药物化学， 2001， 硕士

【摘要】 经过溶剂法和各种色谱方法从十字花科菘蓝属植物板蓝根（Isatis indigotica Fort.）的抗病毒有效部位中分离得到10个化合物，通过谱学分析和理化常数测定并结合文献鉴定了其中7个化合物的化学结构。其中有1个新化合物和6个已知化合物。 新化合物为板蓝根木脂素苷A（Indigoticoside A，3-（4-O-β-D-glucopyranosyl-α-hydroxy-3-methoxy-benzyl）-4-（4-hydroxy-3-methoxy-benzyl）tetrahydrofuran，I-2）。 已知化合物分别属于木脂素类、含硫类、呋喃衍生物类及糖衍生物类成分。它们分别是：（+）-异落叶松树脂醇（（+）-isolariciresinol，I-1），表告依春（epigoitrin，I-10），5-羟甲基糠醛（5-hydroxymethyl-furadehyde，I-4），5-羟甲基糠酸（5-hydroxymethyl-furoic acid，I-5），正丁醇-β-D-吡喃型果糖（n-butyl-β-D-fructopyronoside，I-6），蔗糖（sucrose，I-3）。其中I-1、I-4、I-5、I-6四个化合物为属中首次分离得到。 利用二维核磁共振技术（2D-NMR），尤其通过HMBC谱成功的解决了木脂素苷类结构中取代基和糖的连接位置等问题，并对新化合物I-2的碳氢信号进行了确切归属。 药理实验结果表明，板蓝根提取物经大孔树脂用80％醇洗下的部分和正丁醇萃取部分在体外具有较强的抗病毒活性。更多还原

【Abstract】 Abstract Abstract 10 natural compounds were seperated from the antiviral site of Isatis indigotica Fort. by solvent and chromatography. 7 of them were indentified by spectroscopic methods, physiochemical properties and literature data. Among them, there was one novel compound and six known compounds. The novel compound was 3-.(4-O- ~ -D-glucopyranosyl- ci -hydroxy- 3 -methoxy-benzyl)-4-(4-hydroxy-3 -methoxy-benzyl)tetrahydrofuran (Indigoticoside A, 1-2). The know compounds were respectively: (+)-isolariciresinol (I-i), Epigoitrin (I-i 0), 5-hydroxymethyl-furadehyde (1-4), 5-hydroxymethyl -fttroic acid (1-5), n-butyl- ~ -D-fructopyronoside (1-6), sucrose (1-3). Among them, I-i, 1-4, 1-5, 1-6 were isolated from Isatis genus for the first time. In the course of structural determination, 2D-NIvIIR techniques, especially HMIB C spectrum, were successfiully applied to determine the position of the substitute groups and sugar in the structure of lignan glycoside. 慔 and ?C-signals of novel compound 1-2 were assigned by means of 2D-NMR spectra. The pharmacological test showed that n-butanol extract and 80% ethanol elusion fraction obtained from D,0, polymer adsorption column had strong antiviral effects in vitro.更多还原