【作者】 宋志荣；

【导师】 刘明月；

【作者基本信息】 湖南农业大学 ， 蔬菜学， 2001， 硕士

【摘要】 本文以湘研1号、5号、10号为试材，研究水分胁迫下组织相对含水量（RWC）、丙二醛（MDA）含量、质膜相对透性、保护酶（SOD、POD和CAT）活性、抗坏血酸（AsA）含量、叶绿素（Chl）含量、脯氨酸（Pro）含量等生理指标的变化。结果表明：干旱胁迫下辣椒组织的RWC、AsA含量和Chl含量总是呈现下降趋势；Pro含量增加；而保护酶活性则先升高后降低；质膜相对透性、MDA含量在苗期先降低后增加，在盛花期和结果前期则总是保持增加。不同品种间的差异显示总体上湘研5号保持着相对较高的RWC、Chl含量、AsA含量、Pro含量和保护酶活性（或活性的增幅），相对较低的质膜相对透性、MDA含量，而湘研1号则相反，湘研10号介于湘研1号和湘研5号之间；不同生育期间的差异显示苗期保持相对较高的RWC、Chl含量、AsA含量、Pro含量和保护酶活性，相对较低的质膜相对透性、MDA含量，盛花期则相反，结果前期介于苗期和盛花期之间。这表明湘研5号抵御干旱胁迫的能力相对较强，湘研1号则相对较弱；同样，苗期抵御干旱胁迫的能力相对较强，盛花期则相对较弱。水渍胁迫下，质膜相对透性和MDA含量增加，保护酸（SOD、POD和CAT）活性则先升高后降低，湘研5号保持着较低的质膜相对透性和MDA含量以及相对较高的保护酶活性，湘研10则相反，湘研1号介于湘研10号和湘研5号之间。这表明湘研5号抵御水渍胁迫的能力相对较强，湘研10号抵御水渍胁迫的能力相对较弱。相关分析与主成分分析显示：干旱胁迫下不同品种和不同生育期生理指标的变化具有相对的完整性和独立性。这些生理指标极其变化能够反映不同品种和不同生育期抵御水分胁迫能力的差异。更多还原

【Abstract】 Xiangyan NO.1, Xiangyan NO.5 and Xiangyan NO.10 were used as the materials to study changes of some physiological indexes under water stress. The physiological indexes included relative water content, relative permeability of plasmalemma, MDA content, protective enzyme (SOD, POD and CAT) activities, endogenous antioxidant(endogenous ascorbic acid) content, chlorophyll content and free proline content. The results indicated that under drought stress, the relative water content, AsA content and chlorophyll content always decreased; The free proline content always increased; The protective enzyme (SOD, POD and CAT) activities increased at first and then decreased; The relative permeability of plasmalemma and MDA content at the seedling stage increased after decreasing a little, and the two physiological indexes at the flowering stage and the fruit setting early stage always increased. On the whole, under drought stress, Xiangyan NO.5 kept great relative water content, chlorophyll content, free proline content. AsA content and protective enzyme activities (or increase of activities), and kept little relative permeability of plasmalemma and MDA content. However, Xiangyan NO.1 was opposite. Xiangyan NO.10 lay between Xiangyan NO.1 and Xiangyan NO.5; The seedling stage kept great relative water content, chlorophyll content, AsA content, free proline content and protective enzyme activities and kept little relative permeability of plasmalemma and MDA content. However, the flowering stage was opposite. The fruit setting early stage lay between the seedling stage and the flowering stage. This indicated that among the three cultivars, Xiangyan NO.5 had the greatest ability to resist drought stress while Xiangyan NO.1 had the least ability to do it. it also indicated that among the three different growth-development stages, hot pepper had the greatest ability to resist drought stress at the seedling stage and had the least ability to do it at the fruit setting early stage. Under water-soaked stress, the relative permeability of plasmalemma and MDA content always increased while the protective enzyme (SOD, POD and CAT) activities increased at first and then decreased. Moreover, Xiangyan NO.5 kept little relative permeability of plasmalemma and MDA content and kept great protective enzyme activities. Xiangyan NO. IC had the opposite results. Xiangyan NO.1 lay between Xiangyan NO.5 and Xiangyan NO.10. This indicated that among the three cultivars, XiangyanNO. 5 had the greatest ability to resis1 water-soaked stress, and Xiangyan NO.10 had the least ability to resist water-soaked stress. Tlx variant analysis and principal component analysis indicated the changes of the physiologica indexes of the different cultivars and the different growth-development stages under water stress 2 ? 7 were both complete and independent. These physiological indexes and their changes could be used as basis to identify the water stress resistance of the different cultivars and the different growth-development stages. Song Zbirong (Vegetable culture physiology) Directed by Liu Mlngyue (Professor)更多还原