外湿对造模脾虚小鼠免疫学影响的实验研究

【作者】 刘光华；

【导师】 曲长江；

【作者基本信息】 辽宁中医学院 ， 中医基础理论， 2001， 硕士

【摘要】 本实验对劳倦过度加饮食失节法研制的脾虚小鼠模型、外湿小鼠模型、脾虚加外湿小鼠模型在细胞免疫、体液免疫改变方面进行了比较研究。旨在探讨单纯环境因素——外湿对正常小鼠和脾虚造模小鼠免疫学方面的不同改变和影响，揭示湿邪作用机体后产生损伤的免疫学机理，同时探讨脾虚生湿与外湿的关系。 实验结果表明：三组模型小鼠均出现了体重的下降，淋巴细胞转化率的下降，T细胞亚群CD₄的下降、CD₈的升高，免疫球蛋白IgG、IgM的下降，说明各组模型小鼠均出现了免疫功能降低的表现。三组小鼠模型按淋巴细胞转化率、T细胞亚群CD₄、免疫球蛋白IgG、IgM下降程度由低到高排序，依次为C组，B组，D组；按T细胞亚群CD₈升高程度由低到高排序，依次为D组，B组，C组，说明D组免疫功能降低程度最重，表明外因即外湿与内因即脾虚协同作用，导致免疫功能降低，是机体发病的关键。更多还原

【Abstract】 This experiment is a comparative study on the mice with spleen deficiency modeled by over-strain plus irregular diet, with exopathic dampness, with spleen deficiency plus exopathic dampness on bodies- change of cyto-immunity and humoral immunity The immunological changes and effects of single circumstances factorxopathic dampness on normal mice and spleen-deficiency mice are observed, in order to reveal the immunological mechanism that dampness damage organism after it invaded and probe into the relationship between water retention due to spleen deficiency and exopathic dampness. The results show that modeled mice in three groups of all have the decrease of body weight and immune function, such as lymphocyte transformation rate (LCT), CD4, IgG, 1gM and the increase of CD8. In these modeled mice in three groups, according to the decreasing degree of LCT, CD4, IgG and IgM, the sequence from the lower to the higher is spleen deficiency group(C group), exopathic dampness group(B group), spleen deficiency plus exopathic dampness group(D group); according to the increasing degree of CD8, the sequence from the lower to the higher is D group , B group, C group, explaining the D group is the greatest in the decreasing degree of immune function, which indicates the reduction of immune function by the co-ordinate act of exopathic factor(exopathic dampness) and endopathic factor(spleen deficiency) is the key to the occurrence of disease.更多还原