【作者】 张军；

【导师】 杨庆凯；

【作者基本信息】 东北农业大学 ， 作物遗传育种， 2001， 硕士

【摘要】 大豆孢囊线虫（Heterodera glyciaes Ichinohe）是大豆生产上的毁灭性病害之一。土壤一经感染，则极难防治。化学药剂防治尽管有效，但成本太高，而且还造成环境污染。利用植物本身的抗性，培育抗病品种是目前采用的最经济有效的控制措施。培育抗病品种首先要筛选和鉴定抗源材料。本研究采用抗感大豆孢囊线虫的野生、半野生、栽培大豆在接种和未接种大豆孢囊线虫3号生理小种条件下总酚、类黄酮、绿原酸和游离脯氨酸含量动态变化以揭示其抗SCN₃的生化机制。初步探讨了在SCN₃侵染过程中，抗源生化指标（总酚、类黄酮、绿原酸和游离脯氨酸）的表达情况，整个侵染期动态变化以及它们与抗性关系，目的在于揭示抗源品种抗病的内在规律。从而为抗SCN₃资源的筛选和鉴定及抗SCN₃育种提供理论依据，为研究抗SCN₃的遗传提供参考。试验结果表明，抗感材料间在5个不同时期除了第Ⅰ时期相互之间、第Ⅲ时期BR与BS游离脯氨酸含量增量基本一致以外，其他时期相差显著。抗感材料间在接种与未接种SCN₃时总酚、类黄酮、绿原酸含量增量相互之间在五个不同时期差异都显著。在总酚、类黄酮、绿原酸和游离脯氨酸中类黄酮是鉴定SCN₃最佳生化指标。鉴别SCN₃最佳时期为后期。更多还原

【Abstract】 SCN3 is one of the most destruction disease which is hard to cure when itifected. Though effective, its price and pollution makes chemical medicine unusable. The most effective and economical way as to use the resistance inherent in the plant. The first step of cultivating resistant cultivar is the choosing and identi~抜ng resistant germplasm. Wild, semi-wild and cultivated resistant or susceptible to SCN3 material is used in this study. Total phenolicis, flavonoid, chlorogenic acid and free proline are measured to SCN3 to determine the biochemical mechanism of SCN3 resistance. The invasion process discussed. The aim is to disclose to internal regularity of cultivar resistance, so to lay academic foundation for choosing, identi1~ing and cultivity SeN3 resistant material. The results show: The diffrrence are evident in the later period. The difference are evident in the resistant and susceptible material infected by SCN3. Flavonoid is the best biochemical index in the four biochemical indexes. The best identi1~ing period is the later period.更多还原