急性佐剂性关节炎针刺镇痛后效应的实验研究

【作者】 陈瑾；

【导师】 刘光谱；

【作者基本信息】 成都中医药大学 ， 中西医结合基础， 2001， 硕士

【摘要】 中医临床实践证明，针刺具有确切的镇痛作用，并被广泛地应用于痛证的治疗。在此基础上，进一步发现，针刺镇痛不仅有即时效应，而且可能存在后效应。 本实验研究以急性佐剂性关节炎（AA）大鼠作为炎症痛模型，选取昆仑穴采用传统的手针疗法施治，对针刺治疗1次之后效应进行了观察。实验中用放免、神经生化、原位杂交等检测方法，对炎症局部痛阈，大脑皮质5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）含量，脑干5-HT和5-HIAA含量，炎症局部5-HT和5-HIAA含量，下丘脑β-内啡肽（β-EP）含量以及下丘脑β-EP前体前阿黑皮素POMC mRNA表达等指标进行了观察。 实验结果表明：针刺能提高AA大鼠痛阈并能持续1h以上；提高大脑皮质5-HT和5-HIAA含量并能持续30min以上；提高脑干5-HT和5-HIAA含量并能持续30min以上；降低炎症局部5-HT和5-HIAA含量并能持续30min以上；提高下丘脑β-EP 含量并能持续30min以上；促进下丘脑β-EP 前体POMCmRNA的基因表达并能持续1h以上。 动物实验提示针刺镇痛存在即时效应，也存在显著的后效应，外周和中枢的镇痛系统均参与了这一过程。这一研究结果不仅科学地证明了传统手针方法针刺镇痛后效应的存在，而且为深入探讨针刺镇痛后效应的机理提供了重要的实验依据。更多还原

【Abstract】 The clinical practice of TCM has showed that acupuncture has a specific effectof relieving pains, and then it has been wide1y used in treatment of patients withpainful symptoms. Being based upon this, people also have observed thatacupuncture has not only immediate effect but also aftereffect in relieving pains.In this study, a infiarnmatory-pain model was made in rats with acute adjuvantarthritis. KuIilun point was selected and the traditional method of acupuncturetheraPy was used. Then, the acupunctUre analgesia aftereffectS tbxough one time ofacuPunctUre were observed. The experimental parameters were collectCd byradioimmunoassay neurobiochemisny and in situ hybridization. They included painthreshOld in indamed area; the contents of 5-HT and 5-HIAA in cerebral cortex. inbrain stem and in inflamed area; the contents of fl -EP and the expression of POMCmRNA in hyPothalamus.The results showed that acupuncture had the fOllowing functions:1. Heighened pain threshold, and it lnyted more than one houL2. Raised the contents of 5-HT 5-HIAA in cerallral cortex, and it lasted morethan 30 rninutes.3. Raised the contents of 5-HT 5-HIAA in brain stem, and it lasted more than30 minutes.4. Lowered the contents of 5-HT 5-HIAA in inflamed area, and it lasted morethan 30 minutes.5. Raised the contents of 6 -EP in hypothalamus, and it lasted more than 30minutes.6. Increased the expression of POMC mRNA, and it lasted more than one hourIt suggested that acupuncture had immediate effect and remarkable aftereffectto relieve pains. The peripheral and central 5ystems of pain and analgesicmechanisms were related to this process. The stUdy results not only proved theaftereffect of acupuncture analgesia scientifica1ly but also provided importanteXPerimental evidence for further exploration on its mechanism.更多还原