二至丸对阴虚模型动物神经内分泌免疫网络作用的研究

【作者】 操红缨；

【导师】 梁颂名； 吴清和； 陈淑英；

【作者基本信息】 广州中医药大学 ， 方剂学， 2000， 硕士

【摘要】 二至丸由女贞子、墨旱莲二味药组成，具有益肝肾、补阴血、壮筋骨、乌须发的功效，临床上用于肝肾阴虚所致的口苦咽干、头昏眼花、失眠多梦、腰膝酸软、须发早白等症，其组方简单、药性平和、功效确切，是治疗阴虚证的名方，其滋阴作用的机理已有所探讨，但都是以个别系统的指标为依据，没有从整体水平研究。 阴虚证是指机体精、血、津液等物质亏耗，以及阴不制阳，导致阳相对亢盛，机能虚性亢奋的病理状态。近年来对阴虚证的本质研究取得了一定认识，认为阴虚证不仅与植物神经功能、能量代谢、血液流变学、自由基、血浆环核苷酸、微量元素等方面有关，还与神经内分泌免疫网络调节紊乱有关。 自神经内分泌免疫网络学说提出以来，神经内分泌免疫网络的研究已受到国内外的关注，神经、内分泌、免疫这三大系统在神经、受体及细胞内信息传递系统的基础上彼此交联，相互作用，相互影响，构成神经内分泌免疫网络。神经内分泌免疫网络包括上、中、下三个调节通路，已有大量资料证明，补益类中药复方或某些补益中药的天然活性成分，可作用于神经内分泌免疫网络各调节通路，显示出中医药对神经内分泌免疫网络调控的优势和具有可挖掘的潜力。 本文利用甲状腺素阴虚模型从整体水平，从神经、内分泌、免疫多层次来探讨二至丸调节阴虚证神经内分泌免疫网络紊乱的作用，以进一步探讨二至丸滋阴作用的深层次的机理，结果如下： 1.阴虚大鼠血中皮质醇、胰岛素、睾酮含量下降，T4、胆固醇升高，以及肾上腺、甲状腺、胸腺重量下降，表明阴虚大鼠内分泌机能紊乱是病机中的一个重要环节。实验发现，二至丸能明显提高阴虚大鼠血中皮质醇、胰岛素、睾酮含量以及肾上腺、甲状腺、胸腺重量，降低T4、胆固醇含量。 Z．阴虚大鼠血p．内啡肽含量降低，二至丸升高阴虚大鼠血浆中p－内啡肽的含量，说明其可以提高神经－内分泌－免疫网络的整体机能。 3．阴虚小鼠NKC活性、淋巴细胞增殖能力降低、IL．二诱生能力降低，说明阴虚小鼠的兔疫机能失调，二至丸能提高阴虚小鼠NKC活性、淋巴细胞增殖能力和IL．2诱生能力，从而调节兔疫机能。 4．阴虚大鼠下丘脑、垂体、肝、脾、肾上腺、胸腺的形态学均有改变，二至丸对阴虚大鼠下丘脑、垂体、肝、脾、肾上腺、胸腺的病变均有明显的改善。更多还原

【Abstract】 Since neuroimmuendocrinology(NEI) was founded by Besedovsky, it has been studied wildspreadly in the world. Nerves system, endocrine system and immune system are interwined. There are direct physical links eurons that innervate immune organs, such as spleen and lymph nodes. And now, research are unraveling the molecular links, which include the interleukins, originally viewed only as regulators of immune cells; neurotransmitters, once thought to act only between nerve cells; and hormones, the endocrine messengers. The tonic of Traditional of Chinese Medicine (TCM) has been proved that they can act NET network, which suggested that the TCM has advantage and potential to regulate NET network. The yin syndrome due to deficiency of yin-essence, manifested as low-grade or afternoon fever, flushed cheek, warm palms and soles, night sweet, dry mouth, red lips, red tongue with little coating, small and rapid pulse. The yin syndrome not only with vegetative nerve function, energy metabolism, blood rheology, free radical and microelement, but also with NET network disorder. Pill of Erzhi is composed of Eclipta prostrata L. and Ligustrum lucidum Ait.. It can nourish liver and kidney, blood and yin. It can treat yin syndrome very well. Only independent organ and system have studied the mechanism of Pill of Erzhi, not in whole level. This paper used model of yin syndrome to search its mechanism of regulation of NET network. The result as it follows: The rats of deficiency of yin, their hemotic cortisol, insulin, testosterone decreased; weight of adrenal, thyroid and thymus decreased; cholesterol and thyroid hormone increased, which showed that the rats of deficiency of yin were hyponeuroendocrinism. Pill of Erzhi could improve hemotic cortisol, insulin, testosterone; increased weight of adrenal, thyroid and thymus; reduce hemotic cholesterol and thyroid hormone. Cortisol, insulin, testosterone and thyroid hormone are all take important effect on NET network. y Hemotic ~ -endorphin of the rats of deficiency of yin decreased. ~ - endorphin is an important element in the NEI network. It can improve immune function; regulate neuroendrininism function and et al. Pill of Erzhi could increase P -endorphin of the rat of deficiency ofyin, which may be the factor of its regulating NEI network and treating yin syndrome. The mice of deficiency of yin, their lymphocyte proliferation, activity of NKC and activity of interleukin 2 reduced, which showed that the mice of yin were immunologic inadequacy. Pill of Erzhi could modulate their immunologic inadequacy. It could improve lymphocyte proliferation, activity of NKC and activity of interleukin 2. Tnterleukin 2 not only is an important factor of immune system, but also act of regulating central nevus system and endocrine system. The rats of deficiency of yin, their hypothalamus, pituitary gland, liver, spleen, adrenal and thymus had pathologic change, which showed the rats of deficiency of yin were disorder of NEI network in deferent level. Pill of Erzhi could alter these changes in different level. From all the above results, we can conclude that the yin syndrome is disorder of NEI network. Pill of Erzhi could improve the model of yin syndrome regulating NEI network and keeping homeostasis.更多还原