【作者】 曾庆堂；

【导师】 叶峻； 檀进发； 黄绍明； 柯明华；

【作者基本信息】 广西医科大学 ， 神经解剖学， 2000， 硕士

【副题名】---FG和HRP逆行标记法研究

【摘要】 应用荧光金（Fluoro-Gold，FG）逆行标记法技术研究了大鼠脑黑质的传入纤维联系以及应用荧光金逆行标记法和辣根过氧化物酶（Horseradish Peroxidase，HRP）逆行标记法相结合技术研究了中缝背核、被盖腹侧区、无粒型岛皮质后区向伏核和黑质的分叉投射。将FG泳入一侧黑质，结果在泳入区同侧的额叶、颞叶、顶叶、岛叶、伏核、终纹床核、杏仁中央核、隔核、尾壳核、苍白球、腹侧苍白球、脚内核、丘脑束旁核、下丘脑腹内侧核、结节外侧核、底丘脑核、上丘、臂旁核、中脑中央灰质内侧部和外侧部、被盖腹侧区、小脑间置核；双侧的缰核、穹窿周核、未定带、中缝正中核、中缝背核、艾魏核、脚桥被盖核、蓝斑见到FG逆行标记细胞。将FG泳入一侧黑质，将HRP注入同侧的伏核；结果在双侧的中缝背核和泳入区同侧的无粒型岛皮质后区、被盖腹侧区见到FG和HRP逆行双标记细胞。表明这三个核区有部分细胞分叉投射到伏核和黑质。更多还原

【Abstract】 Afferent fibrous connections of the substantia nigra by a labeling technique of fluorogold(FG) retrograde tracing and dorsal raphe nucleus, ventral tegmental area and posterior agranular insular area arborize to the nucleus ecwnbens and substantia nigra by a double labeling technique of FG retrograde tracing with horseradish peroxidase(HRP) retrograde tracing Inject the FG into one side of the substantia nigra. Following observed the distribution of the retrograde labeled cell, the results were obtained: the FG retrogmd labeled cells were found in the ipsilatcral frontal cortex,temporal cortex, paiietal cortex ,insula, nucleus accumbens, bed nucleus of the siria temiinalis, central amvgdaloid nucleus, septal nucleus, caudate putamen, globiis pallidus, ventral pallidus, entopeduncular nucleus ,parafascrcuJar thalarnic nucleus, ventmlmedial hypolha]amic nucleus ,lateral tubernl nucleus ,supeflor colliculus, lateral part of the mesencephali central gray ,niedial part of the mesencephali central gray, ventral tegmental area,interposed cerebeliar nucleus; bilateral Iiabenular nulceus ,perifomical nucleus .,zona incerta,median raphe nucleus ,dorsal raphe nucleus ,edinger- westphal nucleus ,pedunculoponhine tegmental nucleus ,locus cemleus. Inject the FG into one side of the substantia nigra and inject the HRP into the same side of the nucleus accumbens . Following observed the disiribution of the reirogmde labeled cell. the results ~were obtainedthe double labeled cell with FG and HRP were found in the ipsilateral posterior agranular insular area ,ventral tegmental area and bilateral dorsal raphe nucleus,it is show that collateral projection from each them to the nucleus accumbens and the substanfia nigm.更多还原