【摘要】 建立了5种解热镇痛药中违规添加马来酸氯苯那敏的HPLC-PDA检测方法。采用Eicoee WT C₁₈色谱柱（4.6 mm×150.0 mm,5??m）；流动相为0.02 mol/L庚烷磺酸钠溶液（含1%三乙胺，用磷酸调节pH值至5.5）-乙腈（体积比为70∶30）；流速为1.0 mL/min；光电二极管阵列检测器（Waters 2998,PDA），扫描范围为200～400 nm，检测波长为260 nm，柱温为30℃，进样体积10??L。结果显示，马来酸氯苯那敏的线性范围为0.052～41.500??g/mL（r=0.999 97），安乃近注射液、氨基比林注射液、安痛定注射液、对乙酰氨基酚注射液、双氯酚酸钠注射液回收率分别为100.7%（RSD=0.9%）、98.1%（RSD=0.6%）、98.6%（RSD=0.4%）、97.8%（RSD=0.9%）、99.1%（RSD=0.6%）。该方法分离度良好、灵敏度高、简便准确，可用于常见解热镇痛注射液中违规添加马来酸氯苯那敏的含量检测。更多还原

【Abstract】 The objective was to establish a method for the detection of illegal addition of chlorpheniramine maleate in 5 kinds of antipyretic analgesics. Eicoee WT C₁₈（4.6 mm×150.0 mm, 5 ??m） was used. The mobile phase was 0.02 mol/L sodium heptanesulfonate solution（containing 1% triethylamine, adjusted to pH 5.5 with phosphoric acid）-acetonitrile（70∶30, V∶V）; the flow rate was 1.0 mL/min. PDA detector, scanning range of 200-400 nm, detection wavelength was 260 nm, column temperature was 30℃, and the injection volume was 10 ??L. The linear range of chlorphenamine maleate was 0.052-41.500 ??g/mL（r=0.999 97）. The average recoveries of analgin injection, aminopyrine injection, antonidine injection, paracetamol injection and diclofenac sodium injection were 100.7%（RSD=0.9%）, 98.1%（RSD=0.6%）, 98.6%（RSD=0.4%）, 97.8%（RSD=0.9%） and 99.1%（RSD=0.6%）, respectively. The method has good resolution, high sensitivity,simplicity and accuracy, and can be used for the detection of the content of chlorpheniramine maleate illegally added in common antipyretic analgesic injection.更多还原