【摘要】 为检验治疗猫淋证的自拟中兽药组方“石香通淋散”的抗炎、镇痛、利尿效果，本试验使用吲哚美辛或氢氯噻嗪作为阳性西药对照，复方金钱草作为阳性中药对照，蒸馏水作为阴性对照，采用小鼠耳廓肿胀试验检测抗炎作用，采用小鼠醋酸扭体试验和热板致痛试验检测镇痛作用，采用小鼠代谢笼试验检测利尿作用。二甲苯致小鼠耳廓肿胀试验结果显示，石香通淋散组小鼠的耳廓肿胀程度显著低于阴性对照组和复方金钱草组（P<0.05），与吲哚美辛组相比无显著差异（P >0.05）；石香通淋散组的耳廓肿胀抑制率低于吲哚美辛组，高于复方金钱草组。醋酸诱导小鼠扭体反应试验结果显示，石香通淋散组小鼠的疼痛潜伏期显著长于阴性对照组和复方金钱草组（P<0.05），与吲哚美辛组相比无显著差异（P > 0.05）；石香通淋散组小鼠的扭体次数显著少于阴性对照组和复方金钱草组（P<0.05），显著多于吲哚美辛组（P<0.05）；石香通淋散组的疼痛潜伏期延长率和镇痛率均低于吲哚美辛组，高于复方金钱草组。热板诱导小鼠疼痛反应试验结果显示，在给药后15和30 min，石香通淋散组小鼠的痛阈值提高率显著低于吲哚美辛组（P<0.05），显著高于复方金钱草组（P<0.05）；在给药后60 min，石香通淋散组小鼠的痛阈值提高率显著高于吲哚美辛组和复方金钱草组（P<0.05）；在给药后90 min，石香通淋散组小鼠的痛阈值提高率显著高于复方金钱草组（P<0.05），高于吲哚美辛组，但差异不显著（P > 0.05）。小鼠代谢笼试验结果显示，石香通淋散组小鼠的尿量在给药后1 h显著多于复方金钱草组（P<0.05），在给药后2 h显著少于氢氯噻嗪组（P<0.05），而在给药后3、4、5、6、24 h与2个阳性对照组均无显著差异（P > 0.05）。结果表明，石香通淋散具有良好的抗炎、镇痛和利尿作用，为后续该组方治疗猫淋证的机制研究和中兽医临床应用提供一定的参考。更多还原

【Abstract】 To evaluate the anti-inflammatory, analgesic, and diuretic effects of the self-developed traditional Chinese veterinary formula Shixiang Tonglin Powder for the treatment of feline stranguria syndrome, this study used indomethacin or hydrochlorothiazide as positive controls for Western medicine, Fufang Jinqiancao as a positive control for traditional Chinese medicine, and distilled water as the negative control. The anti-inflammatory effect was assessed using the xylene-induced mouse ear swelling test, analgesic effects were evaluated via the acetic acid-induced writhing test and hot plate test, and the diuretic effect was assessed through the mouse metabolic cage experiment. The results from the ear swelling test showed that the swelling degree of mice in the Shixiang Tonglin Powder group was significantly lower than that of the Negative control and Fufang Jinqiancao groups(P < 0. 05), and not significantly different from the Indomethacin group(P > 0. 05). The inhibition rate of ear swelling in the Shixiang Tonglin Powder group was lower than that of the Indomethacin group and higher than that of the Fufang Jinqiancao group. In the writhing test, the pain latency of the Shixiang Tonglin Powder group was significantly longer than that of the Negative control and Fufang Jinqiancao groups(P < 0. 05), and not significantly different from the Indomethacin group(P > 0. 05). The number of writhes was significantly fewer than that of the Negative control and Fufang Jinqiancao groups(P < 0. 05), but significantly more than that of the Indomethacin group(P < 0. 05). Both the extension rate of pain latency and analgesic rate of the Shixiang Tonglin Powder group were lower than those of the Indomethacin group but higher than those of the Fufang Jinqiancao group. In the hot plate test, at 15 and 30 minutes post-administration, the pain threshold increase rate in the Shixiang Tonglin Powder group was significantly lower than that of the Indomethacin group(P < 0. 05) but significantly higher than that of the Fufang Jinqiancao group(P < 0. 05). At 60 minutes, it was significantly higher than both Control groups(P < 0. 05), and at 90 minutes, significantly higher than the Fufang Jinqiancao group(P < 0. 05) and higher than the Indomethacin group without significant difference( P >0. 05). In the metabolic cage test, the urine volume of mice in the Shixiang Tonglin Powder group was significantly greater than that of the Fufang Jinqiancao group at 1 hour post-administration(P < 0. 05), significantly less than the Hydrochlorothiazide group at 2 hours(P < 0. 05), and not significantly different from either positive control group at 3, 4, 5, 6, and 24 hours(P > 0. 05). In conclusion, Shixiang Tonglin Powder exhibits favorable anti-inflammatory, analgesic, and diuretic effects, providing a theoretical reference for further mechanistic studies and clinical application of this formula in the treatment of feline stranguria syndrome.更多还原