【摘要】 目的 探究300 g/L过氧化氢不同剂量及环境湿度对A2型生物安全柜腔体与排风高效过滤器后端的消毒效果，确定最优应用条件。方法 在安全柜操作腔体8个角及排风高效过滤器后端5个点位，放置嗜热脂肪地芽孢杆菌芽孢菌片与过氧化氢变色指示卡，密封后连接MZ-V200型汽化过氧化氢发生装置。设置80、100、120、140 mL剂量梯度及50.0%～99.9%的6组初始相对湿度梯度，通过菌片培养结合指示卡变色判定消毒效果。结果 120 mL剂量和环境相对湿度≤70%时，经65 min汽化循环消毒，柜内及过滤器后端指示微生物完全杀灭，合格率100%；相对湿度>70%后，过滤器后端菌片阳性数递增，合格率降至66.7%;80、100 mL低剂量组仅腔体消毒合格，过滤器后端合格率分别为79.5%和84.6%。结论 过氧化氢剂量与环境湿度是消毒关键因素，高湿度阻碍消毒剂穿透过滤器，最优条件为120 mL剂量和环境相对湿度≤70%。更多还原

【Abstract】 Objective To investigate the disinfection eff icacy of 300 g/L hydrogen peroxide at different dosages and under various environmental humidity conditions on the chamber and the rear side of the exhaust high-eff iciency f ilter of type A2 biosafety cabinets, and to determine the optimal application conditions. Methods Geobacillus stearothermophilus spore strips and hydrogen peroxide color-change indicator cards were placed at 8 corners of the cabinet’s operating chamber and 5 points on the rear side of the exhaust high-eff iciency f ilter. After sealing, the cabinet was connected to an MZ-V200 vaporized hydrogen peroxide generator. Four dosage gradients(80, 100, 120, 140 mL) and six initial relative humidity gradients(50.0%-99.9%) were set. Disinfection eff icacy was assessed by spore strip culture combined with indicator card color change. Results At a dosage of 120 mL and environmental relative humidity ≤70%, a 65 min vaporization cycle disinfection completely inactivated indicator microorganisms inside the cabinet and on the rear side of the f ilter, with a 100% pass rate. When humidity exceeded 70%, the number of positive spore strips on the rear side of the f ilter increased progressively, and the pass rate dropped to 66.7%. In the low-dosage groups(80, 100 mL), only chamber disinfection was qualif ied, while the qualif ication rates on the rear side of the f ilter were 79.5% and 84.6%, respectively. Conclusion Hydrogen peroxide dosage and environmental humidity are key factors for disinfection. High humidity impedes disinfectant penetration through the f ilter. The optimal conditions are a dosage of 120 mL and environmental relative humidity of ≤70%.更多还原