【摘要】 为科学评价9种杀菌剂在荔枝上应用的安全性，利用高效液相色谱-四极杆飞行时间质谱技术(HPLC-Q-TOF/MS)，建立9种杀菌剂在荔枝全果的多残留分析方法，并采用所建立的分析方法对田间荔枝样品杀菌剂残留量进行分析。匀浆后的样品先加入Qu ECh ERS前处理盐包，再经乙腈提取后，将样品置于全自动Qu ECh ERS样品处理平台振荡10 min进行净化，采用HPLC-Q-TOF/MS对9种杀菌剂进行检测。结果表明，9种杀菌剂在质量浓度0.001～0.1 mg/L内具有较好线性相关性（相关系数R~2均大于0.99），在3个添加水平下(0.005、0.05、0.1 mg/kg)的平均回收率为76.6%～114.8%，相对标准偏差为1.4%～16.4%，方法检出限为1μg/kg，定量限为3μg/kg。吡唑醚菌酯、嘧菌酯、苯醚甲环唑和甲霜灵在荔枝全果中的残留量均低于国家规定的最大残留限制值。研究建立的分析方法可靠简便快速，适于批量样品检测需求。更多还原

【Abstract】 The paper aims to scientifically evaluate the safety of nine fungicides on lychees.A method of the simultaneous detection for 9 fungicides in lychee was established by a high-performance liquid chromatography-tandem quadrupole-time of flight-mass spectrometry (HPLC-Q-TOF/MS).The Qu ECh ERS pre-treatment salt package were firstly added in homogenized samples,and then extracted with acetonitrile.The automated QuEChERS sample processing platform was shaken for 10 minutes to remove impurities of lychee.The data were collected by HPLC-Q-TOF/MS.The calibration curves of 9 fungicides showed good linearity in a concentration range of 0.001-0.1 mg/L (R~2>0.99).The average recoveries were 76.6%-114.8%at three spiked levels (0.005,0.05,0.1 mg/kg),while the relative standard deviations (RSD) were 1.4%-16.4%.The limits of detection and limits of quantification were 1μg/kg and 3μg/kg,respectively.The residue samples were analyzed by using the established detection method.The results showed that the residues of pyrazoxystrobin,azoxystrobin,difenoconazole,and metalaxyl in the whole lychee were all below the maximum residue limit values (MRLs) set by China.The established analytical method is reliable,simple and fast,and suitable for batch samples detection.更多还原