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响应面法优化褐藻胶寡糖酸奶工艺及品质分析
Optimization of Process of Alginate Oligosaccharides Yogurt by Response Surface Methodology and Quality Analysis
【摘要】 为探究褐藻胶寡糖的添加对酸奶品质特性和抗氧化活性的影响,以褐藻胶寡糖和纯牛奶为主要原料制作酸奶,以感官评分和持水力为评价指标,在单因素试验的基础上,通过响应面试验优化酸奶加工工艺,确定褐藻胶寡糖酸奶的最佳发酵工艺,并进行酸奶品质指标测定和抗氧化活性分析。结果表明,褐藻胶寡糖酸奶的最佳发酵工艺为蔗糖添加量5.93%、褐藻胶寡糖添加量0.29%、乳酸菌酸奶发酵剂添加量0.21%、发酵温度41.9℃、发酵时间7.19 h,在此条件下,褐藻胶寡糖酸奶的感官评分较高,pH值、可滴定酸度和乳酸菌总数均符合发酵乳国家标准,黄度值(b~*)增加,色泽偏黄。同时,褐藻胶寡糖的添加提高了酸奶的持水力、硬度、黏聚性、乳酸菌总数及对ABTS自由基和DPPH自由基的清除能力。褐藻胶寡糖的添加提高了酸奶的品质特性和抗氧化活性,同时赋予了酸奶良好的感官品质。该研究为褐藻胶寡糖在酸奶中的应用提供了理论参考。
【Abstract】 In order to investigate the effects of the addition of alginate oligosaccharides on the quality characteristics and antioxidant activity of yogurt, alginate oligosaccharides and pure milk are used as the main raw materials to make yogurt. With sensory score and water holding capacity as the evaluation indexes, based on single factor test, the processing technology of yogurt is optimized through response surface test, the optimal fermentation process of alginate oligosaccharides yogurt is determined, and the quality indexes of yogurt are measured and the antioxidant activity is analyzed. The results show that the optimal fermentation process of alginate oligosaccharides yogurt is 5.93% sucrose, 0.29% alginate oligosaccharides, 0.21% lactic acid bacteria yogurt fermentation agent, fermentation temperature of 41.9 ℃, and fermentation time of 7.19 h. Under these conditions, the sensory score of alginate oligosaccharides yogurt is high, and the pH value, titratable acidity and total number of lactic acid bacteria are all in accordance with the national standard of fermented milk. The yellowness value(b~*) increases, and the color tends to be yellowish. Meanwhile, the addition of alginate oligosaccharides improves the water holding capacity, hardness, cohesiveness, the total number of lactic acid bacteria and ABTS radical and DPPH radical scavenging capacity of yogurt.The addition of alginate oligosaccharides improves the quality characteristics and antioxidant activity of yogurt and endows yogurt with good sensory quality.This study has provided theoretical references for the application of alginate oligosaccharides in yogurt.
【Key words】 alginate oligosaccharides; yogurt; sensory evaluation; water holding capacity; response surface optimization;
- 【文献出处】 中国调味品 ,China Condiment , 编辑部邮箱 ,2025年08期
- 【分类号】TS252.54
- 【下载频次】55