【摘要】 目的：建立苏麻止咳方中迷迭香酸、橙皮苷、射干苷和甘草酸含量的测定方法。方法：采用UPLC法同时测定苏麻止咳方中上述4种成分的含量，色谱柱为Waters ACQUITY UPLC HSS T3（2.1 mm×100 mm,1.8μm）；流动相为0.1%磷酸（A）-乙腈（B），梯度洗脱；流速0.3 mL·min^-1；柱温30℃；检测波长265 nm。结果：迷迭香酸、橙皮苷、射干苷和甘草酸的线性范围分别为1.5～59μg·mL^-1、5～190μg·mL^-1、0.5～19μg·mL^-1、3～116μg·mL^-1，平均加样回收率分别为101.5%、101.7%、102.2%、100.7%,RSD值分别为1.3%、0.9%、1.0%、1.1%。结论：该含量测定方法操作简便、专属性强、重现性好，可用于苏麻止咳方的质量控制。更多还原

【Abstract】 Objective:To establish a method for the simultaneous determination of rosmarinic acid,hesperidin,tectoridin and glycyrrhizic acid in Suma Zhike mixtures by UPLC.Methods:UPLC was performed on a Waters ACQUITY UPLC HSS T3 column （2.1 mm×100 mm,1.8μm）.The mobile phase was0.1%phosphoric acid （A）-acetonitrile （B） with gradient elution.The flow rate was 0.3 mL·min^-1,The column temperature was 30°C.The detection wavelength was set at 265 nm.Results:The linear ranges of rosmarinic acid,hesperidin,tectoridin and glycyrrhizic acid were 1.5-59,5-190,0.5-19 and 3-116μg·mL^-1,respectively,and the average recovery rates were 101.5%,101.7%,102.2%and 100.7%with RSD of 1.3%,0.9%,1.0%and 1.1%,respectively.Conclusion:The method is simple,specific and reproducible and can be applied for the quality control of Suma Zhike mixtures.更多还原