【摘要】 以常用的化学驱油剂石油磺酸盐为研究对象，分别考察了其对土壤中过氧化氢酶、脱氢酶活性、硝化潜势，微生物蛋白质、多糖含量等生化指标以及土壤微生物数量、生长速率、土壤微生物群落等微生物生长特性指标的影响规律，并对其可能的作用机理进行了分析。结果表明：石油磺酸盐对土壤生态环境影响表现出明显的剂量-效应关系，随着石油磺酸盐浓度增加，土壤过氧化氢酶、脱氢酶以及土壤硝化潜势呈现先上升后下降的趋势，总体显示出“低浓度促进，高浓度抑制”的影响规律，而微生物数量与微生物蛋白质、多糖含量则显示出先减少后增加的“低浓度抑制，高浓度促进”的影响规律，同时石油磺酸盐有益于微生物群落的物种丰度增加。石油磺酸盐对土壤生态环境影响的剂量效应研究为石油磺酸盐的合理使用以及保障化学驱环境保护提供了理论依据。更多还原

【Abstract】 Taking the commonly used chemical flooding agent petroleum sulfonate as the research subject, this study investigated its effects on biochemical indicators in soil—including catalase and dehydrogenase activities, nitrification potential, microbial protein, and polysaccharide content—as well as on microbial growth characteristics such as microbial population, growth rate, and community structure. The underlying mechanisms were also analyzed. The results reveal a clear dose-response relationship between petroleum sulfonate and the soil ecological environment. With increasing concentration, soil catalase and dehydrogenase activities and nitrification potential initially rose and then declined, demonstrating an overall “low-concentration promotion, high-concentration inhibition” pattern. In contrast, microbial counts, protein, and polysaccharide content first decreased and then increased, showing a “low-concentration inhibition, high-concentration promotion” trend. Additionally, petroleum sulfonate contributed to increased species richness in the soil microbial community. This study on the dosedependent effects of petroleum sulfonate provides a theoretical basis for its rational use and supports environmental protection in chemical flooding operations.更多还原