【摘要】 通过生理表型检测、转录组测序和蛋白质组学等多种技术，全面鉴定玉米响应F. verticillioides侵染的差异基因(DEGs)和差异蛋白(DEPs)，并进行功能和通路的富集分析。生理指标测定结果显示，除了总叶绿素含量在接种后显著降低外，其余指标均显著升高，表明玉米在受到F. verticillioides侵染后，通过激活一系列生理反应以抵抗病原菌的侵袭。富集分析结果显示，DEGs和DEPs主要富集在亚油酸代谢、苯丙类生物合成、谷胱甘肽代谢、淀粉和蔗糖代谢、氨基糖和核苷酸糖代谢等途径中。基于联合分析结果构建了亚油酸途径中的LOXs基因通过合成JA来增强玉米对F. verticillioides抗性的可能作用模型。通过RT-qPCR和PRM验证14个与致病相关的候选基因和蛋白质。更多还原

【Abstract】 In this study, we comprehensively identified differentially expressed genes(DEGs) and differentially expressed proteins(DEPs) in maize in response to F. verticillioides infection using phenotypic and physiological characteristics, transcriptome sequencing, and proteomics analysis. Physiological characteristics measurement indicated that total chlorophyll content was decreased whereas other characteristics were obviously increased in maize plants,representing maize defense responses conditioning F. verticillioides infection. Enrichment analysis revealed that DEGs and DEPs were primarily enriched in linoleic acid metabolism, phenylpropanoid biosynthesis, glutathione metabolism, starch and sucrose metabolism, and amino sugar and nucleotide sugar metabolism pathways. Based on the results of combined analyses, a potential model was constructed in which LOXs genes in the linoleic acid pathway enhance maize resistance to F. verticillioides through JA synthesis. Fourteen candidate genes and proteins related to pathogenicity were validated by RT-qPCR and PRM.更多还原