【摘要】 WAK蛋白家族成员可直接或间接参与和诱导植物体本身产生防御机制，对植物病原菌的侵入起到抵抗和杀灭的效果。课题组前期研究获得玉米Zm00001eb009320基因，隶属于WAK蛋白家族。为探究该基因在抗病中的作用，通过基因克隆技术，构建Zm00001eb009320基因过表达载体，侵染哥伦比亚野生型的拟南芥植株，筛选得到拟南芥转基因阳性株系，对其进行两代自交种植后，开展抗病性实验。结果表明，接种丁香假单胞菌番茄致病变种(Pseudomonas syringae pv. Tomato DC3000,PstDC3000)后，Zm00001eb009320基因的过表达拟南芥植株体内的水杨酸(SA)信号通路可能被诱导，使得转基因植株对PstDC3000具有一定的抗病效果。更多还原

【Abstract】 Members of the WAK protein family can directly or indirectly participate in and induce plant defense mechanisms against pathogen invasion. The research team previously obtained the maize Zm00001eb009320gene, which belongs to the WAK protein family. In order to explore the role of this gene in disease resistance, this study used gene cloning technology to construct an overexpression vector of Zm00001eb009320 gene, infect Colombian wild-type Arabidopsis plants, screen for transgenic positive Arabidopsis lines, and conduct disease resistance experiments after two generations of self crossing planting. The results showed that after inoculation with Pseudomonas syringae pv. Tomato DC3000(PstDC3000), overexpression of the Zm00001eb009320 gene may induce the salicylic acid(SA) signaling pathway in Arabidopsis plants, making transgenic plants resistant to PstDC3000.更多还原