【摘要】 目的 探讨炎症因子白细胞介素-6(IL-6)在左侧星状神经节(LSG)中慢性过表达对心脏电生理的影响及机制。方法 将16只健康比格犬随机分为IL-6组(n=8)和对照组(n=8)两组，IL-6组对LSG进行多点位微量注射AAV-IL-6,对照组显微注射等量生理盐水，注射前收集血清，测量LSG活性。术前和术后第30天进行24小时动态心电图监测，以记录心脏电生理指标：室性心律失常(VA)发生数量、QT间期、QTc、QT间期离散度、QTc离散度，在术后第30天收集血清并再次测量LSG活性，收集LSG组织进行分子检测。结果 基础状态下，两组心脏电生理指标、血清去甲肾上腺素水平、LSG活性无显著差异。IL-6慢性过表达30 d后，IL-6组VA发生次数显著增加(P<0.05),QT间期、QTc均显著缩短(P<0.05),QT间期离散度、QTc离散度均显著增加(P<0.05),VA评分显著增加。此外，与对照组相比，IL-6组血清去甲肾上腺素水平及LSG活性显著增加，LSG突触素密度显著增加(P<0.05)。结论 LSG中IL-6慢性过表达降低心脏电生理稳定性、增加VA发生次数，可能与LSG重构诱导的自主神经功能紊乱有关。更多还原

【Abstract】 Objective To examine the effects and underlying mechanisms of chronic overexpression of the inflammatory cytokine interleukin-6(IL-6) in the left stellate ganglion(LSG) on cardiac electrophysiological properties.Methods Sixteen healthy Beagles were randomly assigned to two groups: the IL-6 group(n=8) and the control group(n=8).In the IL-6 group, multisite microinjections of AAV-IL-6 were administered to the LSG,while the control group received an equal volume of saline.Prior to injection, serum samples were collected, and LSG activity was measured.A 24-hour ambulatory electrocardiogram monitoring was performed both preoperatively and on postoperative 30 days, including the incidence of ventricular arrhythmias(VA),QT interval, QTc, QT interval dispersion, and QTc dispersion.Serum was again collected, and LSG activity was measured on day 30 post-surgery, with LSG tissues harvested for molecular analysis.Results At baseline, no significant differences were observed between the two groups in terms of cardiac electrophysiological parameters, serum norepinephrine levels, or LSG activity.After 30 days of IL-6 overexpression, the IL-6 group exhibited a significantly higher incidence of VA(P<0.05),along with a marked reduction in both QT and QTc intervals(P<0.05),as well as increased dispersion of both the QT and QTc intervals(P<0.05).Additionally, the IL-6 group showed significantly elevated serum norepinephrine levels and LSG activity compared to the control group, with a notable increase in synaptophysin density in the LSG(P<0.05).Conclusion Chronic overexpression of IL-6 in the LSG compromises cardiac electrophysiological stability and raises the incidence of VA,likely due to LSG remodeling-induced disruption of cardiac autonomic regulation.更多还原