【摘要】 旨在制备鸭坦布苏病毒(DTMUV)E蛋白多克隆抗体并建立抗体检测方法。克隆DTMUV E基因，构建重组质粒pET-32a-E并进行原核表达，对表达蛋白提纯后免疫家兔，制备兔抗DTMUV E蛋白多克隆抗体。以原核表达的E蛋白作为包被抗原，建立了检测抗体的间接ELISA方法。结果：DTMUV E蛋白在大肠杆菌中成功表达，且具有良好的免疫原性，制备的兔源抗DTMUV E蛋白多克隆抗体能够与DTMUV发生良好特异性反应，建立的抗体间接ELISA检测方法具有高敏感性、特异性和重复性。提示：制备的DTMUV多克隆抗体和建立的抗体间接EIISA检测方法为DTMUV的深入研究提供了技术支撑。更多还原

【Abstract】 This study aimed to prepare polyclonal antibodies against the E protein of duck Tembusu virus(DTMUV) and to establish an antibody detection method. The DTMUV E gene was cloned, and a recombinant plasmid, pET-32a-E, was constructed for expressing the protein in prokaryotic cells. The purified expressed protein was used to immunize rabbits, resulting in the production of rabbit polyclonal antibodies against DTMUV E protein. And an indirect ELISA method was developed using the prokaryotically expressed E protein as the coating antigen to detect the presence of antibodies. The results demonstrated successful expression of the DTMUV E gene in Escherichia coli, showing good immunogenicity. The generated rabbit polyclonal antibodies showed a strong reaction with DTMUV, indicating their effectiveness. The established indirect ELISA antibody detection method exhibited high sensitivity, specificity, and reproducibility. The prepared DTMUV polyclonal antibodies and the established indirect ELISA antibody detection method in the present study contributed to the advancement of DTMUV research, serving as valuable technical support.更多还原