【摘要】 克隆红嘴鸥(Larus ridibundus) IFN-α基因，并将其与3种家禽(鸡、鸭、鹅)的 IFN-α基因序列进行比较研究，为后续红嘴鸥IFN-α蛋白的获取及其功能研究提供理论支持。PCR扩增及测序获得红嘴鸥 IFN-α基因全序列，长度为579 bp,与3种家禽的 IFN-α基因在长度和序列相似性上存在差异，而与三趾鸥的亲缘关系最近。序列分析显示，4种IFN-α蛋白均为疏水性蛋白，不存在跨膜区，且均含有信号肽、糖基化位点和磷酸化位点，但位于不同的氨基酸位置。亚细胞定位表明，4种IFN-α蛋白主要分布在细胞核内，但含量有所不同。此外，二级结构和三级结构预测显示，4种IFN-α蛋白均以α-螺旋为主，但位点不同。更多还原

【Abstract】 This study cloned the interferon-α( IFN-α) gene from Larus ridibundus and conducted a comparative analysis with IFN-α gene sequences from three domestic poultry species-chicken, duck, and goose to support future studies on the expression and functional characterization of the IFN-α protein of L.ridibundusn. The full-length IFN-α gene of L.ridibundus, 579 bp long, was successfully obtained using PCR amplification and sequencing. Sequence comparison revealed differences in sequence length and similarity between L.ridibundus and the three poultry species, with L.ridibundus showing a closest phylogenetic relationship to Rissa tridactyla. Protein sequence analysis indicated that all four IFN-α proteins are hydrophobic, lack transmembrane domains, and possess signal peptides, glycosylation sites, and phosphorylation sites, although these functional elements are located at different amino acid positions. Subcellular localization predictions showed that all four proteins are primarily located in the nucleus, albeit with varying distribution levels. Furthermore, secondary and tertiary structure predictions suggested that α-helices are the dominant structural elements across all four IFN-α proteins, although the specific positions of these helices vary.These findings provide foundational data for the recombinant expression and functional investigation of L.ridibundus IFN-α protein in future antiviral studies.更多还原