【摘要】 目的：探讨小檗碱对前列腺癌PC-3细胞多西他赛化疗敏感性的影响。方法：于2025年1—6月，利用前列腺癌PC-3细胞于3～5周雄性裸鼠皮下建立荷瘤模型，将接种部位皮下出现绿豆大小结节的50只裸鼠随机分为单纯化疗组、化疗联合小檗碱组、单纯放疗组、放疗联合小檗碱组和模型组，各10只。实时监测各组裸鼠肿瘤体积及体质量变化，通过基因芯片检测基因表达变化。结果：化疗联合小檗碱组和放疗联合小檗碱组肿瘤体积小于模型组、单纯化疗组及单纯放疗组（P<0.05）；模型组裸鼠体质量高于各干预组（P<0.05）；化疗联合小檗碱组与放疗联合小檗碱组裸鼠体质量均高于单纯化疗组、单纯放疗组（P<0.05）。化疗联合小檗碱组与放疗联合小檗碱组A260/A280比值、A260/A230比值高于模型组、单纯化疗组及单纯放疗组（P<0.05）。结论：小檗碱对前列腺癌PC-3细胞多西他赛化疗敏感性有积极影响，其机制可能与改善肿瘤微环境、调节相关基因表达有关，进而提高抗肿瘤疗效。更多还原

【Abstract】 Objective: To investigate the influence of berberine on docetaxel chemotherapy sensitivity in prostate cancer PC-3 cells. Methods: From January to June 2025, the tumor-bearing models in male nude mice at 3-5 weeks old were established subcutaneously using prostate cancer PC-3 cells. Fifty nude mice with subcutaneous nodules of mung bean size at the inoculation site were randomly divided into chemotherapy alone group, chemotherapy combined with berberine group, radiotherapy alone group, radiotherapy combined with berberine group and model group, with 10 mice in each group. The changes of tumor volume and body weight of nude mice in each group were monitored in real time manner, and the changes of gene expression were detected using gene chip. Results: The tumor volumes in chemotherapy combined with berberine group and radiotherapy combined with berberine group were smaller than those in model group, chemotherapy alone group, and radiotherapy alone group(P<0.05). The body weight of nude mice in model group was higher than that in all intervention groups(P<0.05). The body weight of nude mice in chemotherapy combined with berberine group and radiotherapy combined with berberine group was higher than that chemotherapy alone group, and radiotherapy alone group(P<0.05). The ratios of A260/A280 and A260/A230 in chemotherapy combined with berberine group and radiotherapy combined with berberine group were higher than those in model group, chemotherapy alone group and radiotherapy alone group(P<0.05). Conclusion: Berberine has a positive effect on the docetaxel chemotherapy sensitivity in prostate cancer PC-3 cells, and the mechanism may be related to improving the tumor microenvironment and regulating the expression of related genes, thereby improving the anti-tumor efficacy.更多还原