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海马齿对3种典型赤潮甲藻的化感作用及潜在化感物质的鉴定

Allelopathic effects of Sesuvium portulacastrum on three typical red tide dinoflagellates and identification of potential allelopathic substances

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【作者】 吴克敏; 鲁介一; 黄凌风;

【Author】 Wu Kemin;Lu Jieyi;Huang Lingfeng;Key Laboratory of the Ministry of Education for Coastal Wetland Ecosystems, College of the Environment and Ecology, Xiamen University;

【通讯作者】 黄凌风;

【机构】 厦门大学环境与生态学院,滨海湿地生态系统教育部重点实验室;

【摘要】 本研究旨在探讨海马齿对典型赤潮藻的化感作用及其物质来源。选取典型赤潮藻海洋原甲藻、米氏凯伦藻和塔玛亚历山大藻为受试对象,以滨海耐盐植物海马齿的种植水为研究材料。通过C18固相萃取柱吸附海马齿种植水中的根系分泌物,并采用甲醇、乙酸乙酯、二氯甲烷和正己烷萃取,4种萃取物对3种赤潮甲藻的生长表现出不同程度的抑制,其中二氯甲烷萃取物抑制效果最显著,质量浓度在10 g/L对上述3种藻类的抑制率分别为50.83%、97.30%和81.41%。对其进行气相色谱质谱(GC-MS)分析,共检测出19种脂肪酸及其衍生物。从中筛选出的硬脂酸、油酸酰胺和二十四烷醇均表现出抑藻活性,二十四烷醇的抑藻活性最强,当质量浓度处于2 mg/L时,对上述3种藻类的抑制率分别为90%、100%和81.04%。以上结果显示,海马齿能够释放脂肪酸及其衍生物来抑制赤潮藻生长,具备进一步开发为赤潮防治植物修复工具种的潜力。

【Abstract】 This study aimed to investigate the allelopathic effects of Sesuvium portulacastrum (Aizoaceae) on typical red tide algae and identify the sources of the active compounds.Three red tide algae species,Prorocentrum micans,Karenia mikimotoi,and Alexandrium catenella,were selected as test subjects.The planting water of the coastal salt-tolerant plant Sesuvium portulacastrum served as the research material.Root exudates from the plant were adsorbed using a C18 solid-phase extraction column and then extracted with methanol,ethyl acetate,dichloromethane,and n-hexane.The four extracts exhibited varying degrees of inhibition on the growth of the algae,with the dichloromethane extract showing the most significant inhibitory effect.At a concentration of 10 g/L,the inhibition rates for the three algae species were 50.83%,97.30%,and 81.41%,respectively.Gas chromatography-mass spectrometry (GC-MS) analysis identified 19 fatty acids and their derivatives.Among these,stearic acid,oleamide,and docosanol exhibited algicidal activity,with docosanol showing the strongest effect.At a concentration of 2 mg/L,the inhibition rates for the three algae species were 90%,100%,and 81.04%,respectively.These results indicate that Sesuvium portulacastrum can release fatty acids and their derivatives to inhibit the growth of red tide algae,demonstrating its potential as a plant-based tool for red tide control and environmental remediation.

【基金】 国家重点研发计划(2024YFF1306805)
  • 【文献出处】 海洋学报 ,Haiyang Xuebao , 编辑部邮箱 ,2025年03期
  • 【分类号】X55;X173
  • 【下载频次】20
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