【摘要】 目的 了解湖北省基层血防专业技术人员检测能力，提高全省血吸虫病人群查病现场工作质量。方法 2021-2024年间共计抽取湖北省22个血吸虫病流行县（市、区）30个流行村作为质控考核对象，对人群查病的血清学和病原学检测质量进行考核。采用间接红细胞凝集试验（IHA）进行血清学复核，本地检测为阳性的血清全部进行复核，本地检测为阴性则至少抽查10%进行复核。采用镜检法对改良加藤厚涂片（Kato-Katz片）进行病原学复核，本地检测为阳性的Kato-Katz片全部复核，阴性片至少抽查10%复核。结果 采用IHA法共计复核血清样本1 854份。其中本地检测阳性血清样本数为261份，省级对其全部进行复核，复核阳性数为253份；本地检测阴性血清样本数为11 693份，省级抽查1 593份进行复核，复核阴性数为1 593份。本地检测结果与省级复核结果比较，总符合率为99.57%(1 846/1 854)，阳性符合率为96.93%(253/261)，阴性符合率为100.00%(1 593/1 593)，总误检率为0.50%(8/1 601)，总漏检率为0.00%(0/253)，本地检测结果与省级复核结果差异无统计学意义（χ~2=0.145,P>0.05）。2021-2024年血清学阳性人数共计253人，其中高滴度（滴度≥1∶80）人群占比为4.35%(11/253)。不同年份、不同流行类型和流行程度地区IHA阳性人群血清抗体滴度分布差异均无统计学意义（χ~2=11.015、6.831、2.716,P均>0.05）。不同年份、不同流行类型和流行程度地区IHA高滴度人群占比差异均无统计学意义（χ~2=2.614、1.037、1.150,P均>0.05）。病原学共计复核Kato-Katz片509张，结果均为阴性片，总符合率为100.00%(509/509)。结论 血清学和病原学检测总体符合率较高，部分地区存在误诊情况。各地需持续关注IHA高滴度人群，强化基层血防专业技术人员检测水平。更多还原

【Abstract】 Objective To understand the detection capacity of grassroots level professional personnel involved in schistosomiasis control and prevention of Hubei, and improve the quality of field schistosomiasis detection of the province. Methods 30 schistosomiasis endemic villages was selected as the assessment object from the 22 endemic counties(cities, districts) in Hubei during 2021 to 2024, the quality of serological and pathogenic detection in population were assessed. Indirect haemagglutination test(IHA) was taken to recheck the serum samples. If the samples were tested positive by local detection, there would be all rechecked, and if the samples were negative,10% would be rechecked randomly. The KatoKatz thick smears were rechecked by microscope, and the positive smears would be all rechecked, while the negative smears randomly gather 10% to recheck. Results 1 854 serum samples had been collected to recheck by IHA method. The positive serum samples tested by local detection were 261 servings, and the actual positive numbers were 253 proved by provincial institution. The number of negative serum samples tested by local detection were 11 693 servings. The provincial institution randomly selected 1 593 negative samples to recheck, and it proved all negative. Comparing the results between local detection and provincial retest, the total coincidence rate, positive coincidence rate, negative coincidence rate, total false detection rate and total missing rate were 99.57%(1 846/1 854), 96.93%(253/261), 100.00%(1 593/1 593), 0.50%(8/1 601) and 0.00%(0/253), respectively. There showed no significant difference between the results of local and provincial detection （χ~2=0.145,P>0.05）. A total of 253 serologically positive people were detected during 2021 to 2024. Among them, the people with high antibody titers(higher than or equal to 1∶80) accounted for 4.35%(11/253). The distribution of serological antibody titers of IHA positive people showed no statistically significant differences among different years, different epidemic types and different epidemic degrees districts （χ~2=11.015, 6.831, 2.716;all P>0.05）. The proportions of IHA-high-titers population in different years, different epidemic types and different epidemic degrees districts also showed no significant differences （χ~2=2.614, 1.037, 1.150;all P>0.05）. 509 Kato-Katz thick smears were rechecked, and it proved all negative, with a total coincidence rate of 100.00%(509/509). Conclusions The overall coincidence rate of serological and pathogenic detection was relatively high, and false detection existed in some areas of Hubei. It should continuously pay attention to the IHA-high-titers population, and enhance the detection level of grassroot professional and technical personnel.更多还原