【摘要】 【目的】分析并筛选出祁连圆柏7种挥发物中引诱圆柏大痣小蜂成虫的活性物质，通过林间试验验证其引诱效果，为保护祁连圆柏林地、提高祁连圆柏林木种子质量和林地更新提供科学依据，为圆柏大痣小蜂与寄主植物间的通讯机制探索提供基础。【方法】使用Y型嗅觉仪测定祁连圆柏7种挥发物对圆柏大痣小蜂的引诱和驱避效果，筛选具有较好引诱效果的单体-质量浓度进行复配并用于室内引诱试验，从中选择引诱效果较好的复配配方，再结合诱捕器开展林间诱捕技术研究。【结果】1) 10μg?μL^-1质量浓度下，有5种挥发物的圆柏大痣小蜂雌成虫引诱率极显著高于驱避率，分别为桧烯、2,4-二甲基苯乙酮、α-松油醇、α-环氧蒎烷、月桂烯。1μg?μL^-1质量浓度下，α-环氧蒎烷和月桂烯的引诱率显著高于驱避率。对于雄成虫，10μg?μL^-1质量浓度下，3-蒈烯和月桂烯的引诱率极显著高于驱避率；1μg?μL^-1质量浓度下，2,4-二甲基苯乙酮的引诱率极显著高于驱避率，α-松油醇和α-环氧蒎烷的引诱率显著高于驱避率。2)复配试验中配方G（3-蒈烯+月桂烯+α-环氧蒎烷）对圆柏大痣小蜂雌成虫引诱率最高（57%,P<0.05），其次是配方B（月桂烯+α-环氧蒎烷）和A（月桂烯+3-蒈烯），配方I对圆柏大痣小蜂成虫的驱避率最高。3)林间诱捕试验中，3种复配配方以G、A、B为诱芯的诱捕量均显著高于对照组，配方G的诱捕量最多，占诱虫总数的34.1%，配方A和B分别占诱虫总数的27.3%和25.7%。3种颜色诱捕器中黄色诱捕器引诱效果最好，诱捕到成虫234头，占诱捕总数的56.5%，蓝色和绿色诱捕器分别诱虫82和98头。船型诱捕器诱虫209头，三角形诱捕器诱虫205头，2种诱捕器的诱虫量无显著差异（P>0.05）。【结论】3种复配配方均有林间引诱效果，其中配方G对圆柏大痣小蜂的引诱效果最好，具有成为诱捕器诱芯的潜在能力，配合黄色诱捕器使用效果最佳。实际应用时，可采用配方G作为诱芯搭配黄色诱捕器，对圆柏大痣小蜂进行林间种群监测。更多还原

【Abstract】 【Objective】This study analyzed and screened the active substances in the volatiles of Sabina przewalskii that attract Megastigmus sabinae. By verifying their attractiveness through field experiments, this study aims to provide scientific basis for protecting S. przewalskii forest land, improving the quality of S. przewalskii seeds and regeneration. At the same time, it is also to provide a basis for exploring the communication mechanism between M. sabinae and its host plants.【 Method】 A Y-tube olfactometer was used to determine the attractiveness and repellency of 7 volatiles to M. sabinae. Monomers and their concentrations with optimal attractiveness were selected and their combination was used for indoor attraction experiments. The compound formula with superior attractiveness was identified and then used in conjunction with traps to conduct field trapping technique research.【Result】1) In the monomer experiment, at a concentration of 10 μg?μL^-1, there were five compounds that exhibited significantly higher attraction rates than the repellent rates for female M. sabinae. These compounds were sabinene, 2,4-dimethy lacet ophenone, α-terpineol, alpha-pinene oxide, and myrcene. At a concentration of 1 μg?μL^-1, the attraction rates of alphapinene oxide and myrcene were significantly higher than the repellent rates. For male adults, at a concentration of 10 μg?μL^-1, the attraction rates of 3-carene and myrcene were significantly higher than the repellent rates. At a concentration of 1 μg?μL^-1, the attraction rate of 2,4-dimethylacetophenone was significantly higher than the repellent rate, while the attraction rates of α-terpineol and alpha-pinene oxide were significantly higher than the repellent rates. 2) In the compound experiment, formula G had the highest attraction rate for female M. sabinae（57%, P<0.05）, followed by formulas B and A. Formula I had the highest repellent rate for M. sabinae. 3) In the field trapping experiment, traps with compound formulas G, A and B as lure cores had significantly higher trapping volumes than the control group. Formula G had the highest trapping volume, accounting for 34.1% of the total number of trapped insects. Formulas A and B accounted for 27.3% and 25.7% of the total number of trapped insects, respectively.Among traps of three different colors, yellow traps had the best attraction effect, trapping a total of 234 adult insects, accounting for56.5% of the total number of trapped insects. Blue and green traps trapped 82 and 98 insects, respectively. The boat-shaped trap trapped 209 insects while the triangular trap trapped 205 insects. There was no significant difference in trapping volume between these two types of traps（P>0.05）. 【Conclusion】All three formulas demonstrates an attractive effect in the field, with formula G exhibiting the greatest attraction effect on M. sabinae and showing potential as a lure core for traps. The most effective results are achieved when used in conjunction with yellow traps. Thus, in practical applications, formula G can be used as a core paired with a yellow trap to monitor the population of M. sabinae in the forest.更多还原