【摘要】 玉米矮花叶病毒(Maize dwarf mosaic virus, MDMV)是一种重要的检疫性病毒，严重影响玉米的生产。为了有效防治玉米矮花叶病，本研究基于反转录重组酶介导等温核酸扩增(RT-RAA)技术和CRISPR/Cas12a系统构建了一种MDMV快速检测方法，将所有试剂集中在一个试管中进行反应，并通过侧向流动试纸条(LFD)检测反应结果。本研究筛选得到的最佳反应条件为，引物添加量2.8μL、FB报告分子浓度100 nmol/L、RT-RAA系统反应时间20 min、CRISPR/Cas12a系统反应时间20 min。本研究构建的基于RT-RAA和CRISPR/Cas12a的一管式玉米矮花叶病毒快速检测方法特异性强、灵敏度高，且所用试验材料方便携带和运输，适用于现场检测。更多还原

【Abstract】 Maize dwarf mosaic virus(MDMV) is an important quarantine virus that seriously affects maize production. To effectively prevent and control maize dwarf mosaic disease, this study constructed a rapid detection method for MDMV based on reverse transcription-recombinase aided amplification(RT-RAA) technology and the CRISPR/Cas12a system. All reagents were reacted in a single test tube, and the reaction results were detected by lateral flow dipstick(LFD). The optimal reaction conditions screened in this study were as follows: primer addition amount of 2.8 μL, FB reporter molecule concentration of 100 mol/L, RT-RAA system reaction time of 20 minutes, and CRISPR/Cas12a system reaction time of 20 minutes. The one-tube rapid detection method for maize dwarf mosaic virus based on RT-RAA and CRISPR/Cas12a constructed in this study has strong specificity, high sensitivity, and the experimental materials used are convenient for carrying and transportation, making it suitable for on-site detection.更多还原