【摘要】 目的：探讨环黄芪醇(CAG)对脂多糖(LPS)诱导小鼠成肌细胞(C2C12)炎性损伤所致衰老的保护作用及机制。方法：培养C2C12细胞，分为4组：对照组、CAG组、LPS组、LPS+CAG组，适宜的浓度和时间进行干预。显微镜和电镜对细胞形态进行观察。CCK8法检测细胞增殖。取细胞培养上清液，采用ELISA法检测白细胞介素(IL)-1β的表达水平。取细胞沉淀，提取蛋白和RNA,TBA法检测丙二醛(MDA)水平，羟胺法检测超氧化物歧化酶(SOD)水平，Western印迹、PCR技术检测成肌细胞增殖和分化相关蛋白和基因表达水平，如MYOD、MYOG等；还有Hippo信号通路中关键蛋白哺乳动物不育系20样激酶1/2(Mst1/2)、大肿瘤抑制基因1/2(LATS1/2)、Yes相关蛋白(YAP)的表达情况。结果：100μmol/L LPS干预后，C2C12细胞有衰老的表现，MDA水平增加，SOD水平下降，细胞增殖分化受到抑制；Western印迹试验、聚合酶链式反应(PCR)等检测技术表明，MYOD、MYOG表达量降低(P<0.05);Mst1/2、LATS1/2、pYAP表达量增加。经过20μmol/L CAG的治疗后，C2C12细胞衰老的症状减轻，MDA水平下降，SOD水平上调，细胞增殖分化受到抑制的情况逆转；WB、PCR等检测技术表明，MYOD、MYOG表达量增加(P<0.05);而Mst1/2、LATS1/2、pYAP表达量下降，Hippo信号通路被抑制。结论：CAG对LPS诱导C2C12细胞炎性损伤所致衰老具有保护作用，其作用机制可能是通过调控Hippo信号通路实现的。更多还原

【Abstract】 Objective To explore the protective effect and mechanism of cycloastragalus on aging induced by LPS-induced inflammatory injury in C2C12 cells. Methods C2C12 cells were cultured and divided into four groups: control group, cycloastragalus group, LPS group, and LPS + CAG group. Applying the intervention at the appropriate concentration and time.Using microscopes and electron microscopes to observe cell morphology. Using the CCK8 method to detect cell proliferation, ELISA was used to detect the expression level of IL-1β in the supernatant of the cell culture. After cell precipitation, protein and RNA were extracted, malondialdehyde(MDA)levels were detected by the TBA method, Super Oxide Dismutase(SOD)levels were detected by the hydroxylamine method, and expression levels of proteins and genes related to myoblast proliferation and differentiation, such as MYOD and MYOG, were detected by Western Blot and PCR. The expression of key proteins Mst1/2, LATS1/2, and YAP in the Hippo signaling pathway was also detected. Results After intervention with 100 μmol/L LPS, C2C12 cells exhibited signs of senescence, characterized by increased MDA levels, decreased SOD levels, and inhibited cell proliferation and differentiation. Detection techniques such as WB and PCR indicated that the expression levels of MYOD and MYOG decreased(P<0.05), while the expression levels of Mst1/2, LATS1/2, and pYAP increased. After treatment with 20 μmol/L cycloastragalus, the senescence symptoms of C2C12 cells were alleviated, MDA levels decreased, SOD levels were upregulated, and the inhibition of cell proliferation and differentiation was reversed. Detection techniques such as WB and PCR indicated that the expression levels of MYOD and MYOG increased(P<0.05).Conclusions Cycloastragalus has a protective effect on aging induced by LPS-induced inflammatory injury in C2C12 cells, and its mechanism may be realized by regulating the Hippo signaling pathway.更多还原