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Imaging and dynamic monitoring of aging mitochondria using a two-photon nonlinear structured illumination microscope

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【作者】 黎昕然； 王美婷； 杜鹏； 郑晓敏； 陈嘉杰； 王煜烨； 屈军乐； 李宁； 邵永红；

【Author】 Xinran Li;Meiting Wang;Peng Du;Xiaomin Zheng;Jiajie Chen;Yuye Wang;Junle Qu;Ning Li;Yonghong Shao;College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University;School of Mechanical and Electrical Engineering, Guangdong University of Science and Technology;The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College,Nanchang University;

【通讯作者】 李宁;邵永红;

【机构】 College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University； School of Mechanical and Electrical Engineering, Guangdong University of Science and Technology； The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College,Nanchang University；

【摘要】 Mitochondrial dynamics critically regulate cellular aging. Two-photon nonlinear structured illumination microscopy(TPSIM), a low-phototoxicity live-cell imaging technique, was employed to dynamically track mitochondrial changes in senescent H9C2 cardiomyocytes. System validation in COS7 cells achieved 82-nm resolution, threefold higher than conventional microscopy, and sustained 5-min dynamic imaging. Compared to normal cells, senescent cells exhibited fragmented mitochondria. TP-SIM further captured impaired mitochondrial fusion dynamics during senescence through continuous imaging, demonstrating its dual capability for subcellular-resolution visualization and prolonged organelle tracking in live cells.

【Abstract】 Mitochondrial dynamics critically regulate cellular aging. Two-photon nonlinear structured illumination microscopy(TPSIM), a low-phototoxicity live-cell imaging technique, was employed to dynamically track mitochondrial changes in senescent H9C2 cardiomyocytes. System validation in COS7 cells achieved 82-nm resolution, threefold higher than conventional microscopy, and sustained 5-min dynamic imaging. Compared to normal cells, senescent cells exhibited fragmented mitochondria. TP-SIM further captured impaired mitochondrial fusion dynamics during senescence through continuous imaging, demonstrating its dual capability for subcellular-resolution visualization and prolonged organelle tracking in live cells.

【关键词】 mitochondrial dynamics； live cell super-resolution imaging； structured illumination microscope； cardiac aging；
【Key words】 mitochondrial dynamics； live cell super-resolution imaging； structured illumination microscope； cardiac aging；
【基金】 supported by the Shenzhen Medical Research Fund (No. D2401012);the National Natural Science Foundation of China (Nos. 62275168, 62275164, 62204253;82360055);the Guangdong Natural Science Foundation and Province Project (Nos. 2021A1515011916 and 2023A1515012250);the Foundation from Department of Science and Technology of Guangdong Province (No.2021QN02Y124);the Foundation from Department of Education of Guangdong Province (No. 2023ZDZX2052);the Medical-Engineering Interdisciplinary Research Foundation of Shenzhen University (No. 2023YG002);the Scientific Instrument Developing Project of Shenzhen University (No.2023YQ008);the Shenzhen University 2035 Program for Excellent Research (Nos. 2024C012 and 2024C013);the Characteristic Innovation Projects of Provincial Department of Education (No. 2024KTSCX190)
  • 【文献出处】 Chinese Optics Letters ,中国光学快报(英文版) , 编辑部邮箱 ,2025年09期
  • 【分类号】Q2-33;O439
  • 【下载频次】6
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