【摘要】 目的基于高通量测序技术探讨变应性鼻炎(AR)儿童肠道菌群组成及代谢物的改变。方法 选取2023年12月至2024年5月在汕头大学医学院龙岗妇幼临床学院(深圳市龙岗区妇幼保健院)儿科门诊确诊的11例学龄前期AR儿童作为AR组,选取同期进行体检的21例同龄健康儿童作为对照组。采集两组粪便标本,提取DNA并扩增。采用高通量测序技术分析肠道微生物基因的组成,比较两组肠道菌群组成及代谢产物的差异。结果 与对照组比较,AR组Shannon指数更高,变形菌门和糖菌门丰度明显升高,韦荣菌属、肠球菌属、大肠埃希菌/志贺菌属、嗜血杆菌属、链球菌属相对丰度明显更高,毛旋藻属、厌氧棒状菌属、肠杆菌属、厌氧丁酸菌属、真杆菌属相对丰度明显更低,差异有统计学意义(P<0.05)。两组肠道菌群代谢物主成分比较,差异有统计学意义(P<0.05),最终确定了2 540种差异代谢物,其中AR组1 185种代谢物明显增多,20种代谢物明显减少,1 335种代谢物无明显变化。变量权重值(VIP)分析结果显示共有30种显著差异代谢物,与对照组比较,AR组2种粪便差异代谢物明显减少,具体为3-呋喃甲醇葡萄糖苷和膜海鞘素A。结论 学龄前期AR儿童肠道菌群发生明显改变,与炎症和免疫功能相关。更多还原

【Abstract】 Objective To investigate the changes in the gut microflora(GM) composition and metabolites in children with allergic rhinitis(AR) based on high-throughput sequencing technology.Methods From December 2023 to May 2024,11 preschool children with allergic rhinitis(AR) who visited the pediatric outpatient department of Shantou University Medical College Longgang Maternal and Child Health Hospital Clinical College(Longgang District Maternal and Child Health Hospital) were selected as the AR group,and 21 healthy children of the same age who underwent physical examinations in the child health care department during the same period were selected as the control group.Fecal samples were collected from both groups,and DNA was extracted and amplified.High-throughput sequencing technology was used to analyze the composition of gut microbial genes,and the differences in gut microbiota composition and metabolites between the two groups were compared.Results Compared with the control group,the AR group had a higher Shannon index,with a significant increase in the abundance of Proteobacteria and Saccharibacteria.The relative abundance of Veillonella,Enterococcus,Escherichia coli/Shigella,Haemophilus,and Streptococcus was significantly higher,while the relative abundance of Lachnospira,Anaerostipes,Enterobacteriaceae,Anaerobacterium,and Eubacterium was significantly lower(P<0.05).There were statistically significant differences in the principal components of GM metabolites between the two groups(P <0.05).A total of 2 540 different metabolites were identified,of which 1 185 metabolites were significantly increased,20 metabolites were significantly decreased,and 1 335 metabolites showed no significant changes.Variable importance in projection(VIP) analysis revealed 30 significantly different metabolites.Compared with the control group,2 fecal metabolites were significantly downregulated in the AR group,specifically 3-furanmethanol glucoside and membrane heptosyl A.Conclusion The gut microbiota of preschool children with AR has undergone significant changes,which are related to inflammation and immune function.更多还原