【摘要】 采用浸渍-重结晶法将氯霉素负载于细菌纤维素上，制备了细菌纤维素-氯霉素（BC-Chl）复合膜，采用扫描电子显微镜（SEM）、傅里叶红外光谱（FT-IR）和X射线衍射（XRD）对复合膜的形貌和结构进行了表征，结果表明BC-Chl复合膜保持了细菌纤维素的多孔三维网状结构，氯霉素的负载没有改变细菌纤维素的晶型，但负载过程会在一定程度上破坏纤维素的结晶区。BC-Chl复合膜吸水溶胀12 h后，溶胀比在400%以上，显示出较好的持水性。体外药物释放实验表明，相比于氯霉素原料仅45 min的浓度达峰时间，BC-Chl复合膜的药物浓度达峰时间延长至约2 h，表现出延缓药物释放的作用。抑菌圈试验表明，当载药量约为200μg/mg时，BC-Chl复合膜对谷氨酸棒状杆菌和大肠杆菌的抑菌圈直径比分别为5.8和5.1。使用载药量约为200μg/mg的BC-Chl复合膜处理SH-SY5Y细胞24 h后，细胞活性在75%以上，表明BC-Chl复合膜的细胞毒性较低，生物相容性较好。更多还原

【Abstract】 Bacterial cellulose-chloramphenicol(BC-Chl) composite membranes have been prepared using an impregnation-recrystallization method by loading chloramphenicol on bacterial cellulose. The morphology and structure of the composite membranes were characterized by scanning electron microscopy(SEM), Fourier transform infrared spectroscopy(FT-IR) and X-ray diffraction(XRD). The results show that the BC-Chl composite membrane maintains the porous three-dimensional network structure of bacterial cellulose. The loading of chloramphenicol did not change the crystal form of the bacterial cellulose, but the loading process did destroy the crystalline region of the cellulose to a certain extent. After swelling for 12 h, the swelling ratio of the BC-Chl composite membrane was above 400%, showing its good water retention capacity. In vitro drug release experiments showed that the drug concentration peak time of the BC-Chl composite membrane was extended to about 2 h compared with 45 min for the chloramphenicol raw material, confirming a delayed drug release effect. Inhibition zone tests showed that when the drug loading was about 200 μg/mg, the inhibition zone diameter rates of the BC-Chl composite membrane against Corynebacterium glutamicum and Escherichia coli were 5. 8 and 5. 1, respectively. After SH-SY5Y cells were treated with the BC-Chl composite membrane with a drug loading of about 200 μg/mg for 24 h, the cell activity remained above 75%, indicating that the BC-Chl composite membrane had low cytotoxicity and good biocompatibility.更多还原