【摘要】 为探究MS-222对异育银鲫(Carassius auratus gibelio)麻醉效果、复苏效果以及抗氧化酶活性的影响，采用7个不同浓度(50、60、70、80、90、100和110 mg/L)MS-222对异育银鲫(5.88±0.78)g进行麻醉，通过记录麻醉分期及其时间、鳃中抗氧化酶活性和相关基因的表达，确定异育银鲫的最适MS-222麻醉浓度和时间。结果表明：在MS-222浓度为60、70、80、90和100 mg/L时，异育银鲫均可进入麻醉第Ⅰ期和第Ⅱ期，均能在10 min内复苏；浓度低于50 mg/L时，异育银鲫难以进入深度麻醉；浓度高于100 mg/L时，异育银鲫累计死亡率为40%。选择MS-222浓度为60和100 mg/L进行麻醉，进一步分析不同麻醉剂浓度对鱼体的潜在氧化损伤。100 mg/L MS-222深度麻醉异育银鲫(约4 min)后，鳃中GSH活性和MDA含量均显著上升(P<0.05);HSP70、keap1、PIK3A1、nrf1、MAPK3和caspase3的mRNA相对表达量显著上调(P<0.05);放入清水复苏6 h时，鳃中POD活性显著下降(P<0.05),caspase3和LYZ基因表达量显著上调(P<0.05),异育银鲫逐渐恢复到麻醉前水平；60 mg/L MS-222深度麻醉异育银鲫(24 h)后，鳃中GSH活性上升(P<0.05);NQO、keap1、PIK3A1、Nrf1和MAPK3基因表达量显著上调(P<0.05),放入清水复苏1 min后，所测指标与麻醉前差异不显著(P>0.05)。异育银鲫运输过程中适宜的MS-222浓度为60～100 mg/L,科研试验中MS-222较适宜的浓度为100 mg/L。更多还原

【Abstract】 In order to investigate the effects of MS-222 on anesthetic level, recovery rate and antioxidant enzyme activity of Carassius auratus gibelio(5.88±0.78 g), seven different concentrations of MS-222(50,60,70,80,90,100 and 110 mg/L) were set. The optimal anesthesia concentration and time of MS-222 to C. gibelio were determined based on the anesthesia stage and time, activities of antioxidant enzymes in the gill and mRNA expression of antioxidant-related genes. The result showed that C. gibelio could enter the stage I and stage II of anesthesia while anaesthetized at concentrations of 60, 70, 80, 90 and 100 mg/L, and could recover within 10 min. C. gibelio could not enter deep anesthesia while the anesthetic concentration lower than 50 mg/L, and the cumulative mortality was 40% while the concentration higher than 100 mg/L. Furthermore, two concentrations of MS-222 at 60 mg/L and 100 mg/L were set, and C. gibelio exposed for different time to study the oxidative damage of MS-222 to gills. Results showed that deep anesthesia with 100 mg/L MS-222(about 4 min), GSH activity and MDA content in gills of C. gibelio were significantly increased(P<0.05). The mRNA relative expression of HSP70, keap1, PIK3A1, nrf1, MAPK3 and caspase3 were significantly up-regulated(P<0.05). The POD activity, and mRNA relative expression of caspase3 and LYZ in the gills were recovered to the basal level after 6 h of recovery. After deep anesthesia with 60 mg/L MS-222(about 24 h), GSH activity in gills increased and POD decreased significantly(P<0.05). The transcription of genes NQO, keap1, PIK3A1, Nrf1, MAPK3 and caspase3 were significantly up-regulated(P<0.05). After 1 min of recovery within water, there was no significant difference with them before anesthesia. This study suggested that the appropriate concentration of MS-222 in the transport process of C. gibelio is more than 60 mg/L, and the concentration in scientific researches is less than 100 mg/L.更多还原