【摘要】 建立离子色谱-抑制电导检测法测定化学对照品中酒石酸盐的含量。以酒石酸沃尼妙林对照品为例，采用Dionex IonPac^TM AS19（4×250 mm）阴离子交换色谱柱和Dionex IonPac^TM AG18（4×50 mm）保护柱，以22 mmol/L氢氧化钾溶液为流动相，流速为1.0 mL/min等度洗脱，抑制电导检测器检测，测定酒石酸盐中酒石酸的含量。结果表明，该方法在1.0～50μg/mL浓度范围内线性关系良好（r=0.999）,定量限为0.5μg/mL,酒石酸沃尼妙林在3个水平的加样回收率分别为98.79%、98.91%与96.50%,平均回收率为98.07%（RSD=2.20%,n=9）。用此方法测定3个厂家共5批样品，酒石酸沃尼妙林中酒石酸含量的数值与理论值基本一致。本文建立的离子色谱法准确、灵敏，可用于酒石酸盐成盐比例与含量的测定。更多还原

【Abstract】 To establish an ion chromatography method with suppressed conductivity detection for the tartaric acid determination in the chemical reference standards the tartaric acid content of valnemulin hydrogen tartrate was determined by an Dionex IonPac^TM AS19（4×250 mm） anion exchange column and Dionex IonPac^TM AG18（4×50 mm） guard column, using suppressed conductivity detector. An isocratic eluent of 22 mmol/L KOH was chosen as mobile phase, flow rate was 1.0 mL/min. Results manifested that a good linear relationship was observed within the range of 1.0～50.0 μg/mL（r=0.999, n=6）. The limit of quantification was 0.5 μg/mL. The average spiked recovery was 98.07% at three concentration levels with RSD of 2.20%（n=9）. Five batches of in valnemulin hydrogen tartrate samples from 3 manufactures were tested using this validated method, and the contents of tartaric acid were basically consistent with the theoretical values. The method is accurate and sensitive, which provides a reliable analytical method for the control of the proportion and content of the tartaric acid salts.更多还原