【摘要】 目的 建立一种基于液相色谱串联四极杆质谱联用法同时检测定量干血斑中伏立康唑、泊沙康唑、艾沙康唑的方法。方法 将含有10 μL末梢血的干血斑完整转移至试管中，经含有同位素内标的70%乙腈水溶液萃取，采用C18反相色谱柱作为固定相，含0.1%甲酸的超纯水和甲醇作为流动相进行色谱分离，采用电喷雾正离子电离，在多反应监测模式下进行检测。结果 伏立康唑、泊沙康唑在0.050～2.5 μg·mL^-1内，艾沙康唑在0.10～5.0 μg·mL^-1内与测定值线性关系良好，线性相关系数（r）均大于0.99；各浓度的批内与批间精密度RSD<5.3%，提取回收率为92.1%～104.3%；伏立康唑和泊沙康唑的检测限均为0.050 μg·mL^-1，艾沙康唑的检测限为0.10 μg·mL^-1；在45℃极端环境保存7 d的条件下稳定。结论 该方法可同时对三种抗真菌药物进行准确、精密的定量检测；干血斑样本稳定性好，能够在非温控条件下采用普通快递进行运输，适用于远程医疗中的样本采集和运输，有效降低医疗成本，提高药学服务的可及性。更多还原

【Abstract】 Objective To simultaneously quantify voriconazole,posaconazole,and isavuconazole in dry blood spots with liquid chromatography-tandem triple quadrupole mass spectrometry （UPLC-MS/MS）.Methods Dry blood spots of 10 μL capillary blood were transferred intactly to tubes and extracted with 70% acetonitrile water solution containing isotopic internal standards.Chromatographic separation conducted on C18 reversed-phase column with 0.1% formic acid in water and methanol as the mobile phase.Performed with positive electrospray ionization in multiple reaction monitoring mode was used in the detection.Results Voriconazole and posaconazole at 0.050～2.5 μg·mL^-1,and isavuconazole at 0.10～5.0 μg·mL^-1 showed good linearity （correlation coefficients r>0.99）.The intra-batch and inter-batch precision RSD at all concentrations <5.3%,and spiked recoveries ranged from 92.1% to 104.3%.The limits of detection were all 0.050 μg·mL^-1 for voriconazole and posaconazole,and 0.10 μg·mL^-1 for isavuconazole.The analytes were stable under extreme conditions at 45℃ for 7 days.Conclusion The three antifungal drugs are quantified with high accuracy and precision with the present method.Dry blood spot samples are stable,allowing for fast transportation without temperature-controlled.It is feasible for remote sample collection and transportation,this reducing healthcare cost and improving the accessibility of pharmaceutical service.更多还原