【摘要】 目的 研究香附Cyperi Rhizoma的化学成分及其抗炎活性。方法 利用硅胶、Sephadex LH-20和制备型正相HPLC对香附95%乙醇提取物进行分离纯化，根据理化性质及波谱数据鉴定所得化合物的结构。以脂多糖诱导的小鼠小胶质细胞BV-2炎症模型评价其抗炎活性。结果从香附中分离得到15个化合物，鉴定为：24-methylenecycloartenone(1)、cyperenal(2)、patchoulenone(3)、4,5,6,7,8,8a-六氢-3,4,8,8-四甲基-1H-3a,7-亚甲基甘菊环-4-醇甲酸酯（4）、4-烯-广藿香醇（5）、cyperenol(6)、cyperolactone(7)、（-）-caryophyllene oxide(8)、humuleneepoxideⅡ（9）、humulenediepoxide A(10)、α-tocopherol(11)、litseachromolaevane A(12)、hyperhubein G(13)、nootkatone(14)和mustakone(15)。化合物4和11可显著抑制NO的释放，抑制率分别为（49.17±0.01）%和（51.91±0.05）%。结论 本文首次使用制备型正相HPLC技术对香附石油醚层进行系统分离，化合物2、6、9～11和13为首次从莎草科植物中分离得到，化合物12为首次从莎草属植物中分离得到，化合物4、5和7为首次从香附中分离得到。化合物4和11有较强的体外抗炎活性。更多还原

【Abstract】 Objective To determine the chemical constituents of Cyperi Rhizoma and their antiinflammatory activities. Methods The 95% ethanol extract was separated and purified by silica gel, Sephadex LH-20 and preparative normal phase HPLC. The structures of the compounds were identified according to their physicochemical properties and spectral data. Their anti-inflammatory activities were evaluated by lipopolysaccharide induced BV-2 microglial cell model. Results Totally 15 compounds were isolated and identified as follows: 24-methylenecycloartenone(1), cyperenal(2), patchoulenone(3), 3, 4, 8, 8-tetramethyl-4, 5, 6, 7, 8, 8a-hexahydro-1H-3a, 7-methanoazulen-4-ol(4), patchoulan-4-ene-6-ol(5), cyperenol(6), cyperolactone(7),(-)-caryophyllene oxide(8),humulene epoxide Ⅱ(9), humulene diepoxide A(10), α-tocopherol(11), litseachromolaevane A(12), hyperhubein G(13), nootkatone(14), and mustakone(15). Compounds 4 and 11 significantly inhibited the release of nitric oxide, and the inhibition rates were(49.17±0.01)% and(51.91±0.05)%,respectively. Conclusion The preparative normal phase HPLC has been used for the first time to systematically separate the petroleum ether layer of Cyperi Rhizoma. Compounds 2, 6, 9 ～ 11 and 13 have been isolated from the Cyperaceae family for the first time, compound 12 isolated from the genus Cyperus, and compounds 4, 5 and 7 isolated from Cyperi Rhizoma for the first time.Compounds 4 and 11 exhibit strong in vitro anti-inflammation activities.更多还原