【摘要】 目的：探究鸢尾素对新生大鼠高胆红素血症细胞凋亡的影响。方法：将96只新生SD大鼠随机分为对照组（N组）和模型组（M组），于7和10日龄腹腔注射胆红素溶液进行造模。造模成功后将M组随机分为T0组、T1组、T2组、T3组。T1、T2、T3组分别侧脑室注射40、60和80μg/kg的鸢尾素溶液，余各组注射等量磷酸盐缓冲液溶液。侧脑室注射24h后，神经行为学、苏木精-伊红染色（Hematoxylin-Eosin staining,HE）、原位末端标记法（terminal-deoxynucleoitidyl transferase mediated nick end labeling,Tunel）检测海马区病理改变并统计凋亡率，Western Blot检测海马Bcl-2、Bax和Caspase-3蛋白相对表达量。结果：侧脑室注射鸢尾素后。平面翻正反射：T3组所需时间少于T0、T1、T2组且多于N组（P<0.01）；负趋地性反射：T3组所需时间少于T0、T1、T2组（P<0.01,P<0.05）。HE染色：M组海马神经细胞核固缩、裂解，排列紊乱。T1、T2、T3组细胞形态等不同程度改善。Tunel染色：M组Tunel+细胞增多，布满视野；N组Tunel+细胞少，呈零星分布；T3组凋亡率低于T0、T1组（P<0.05）。Western Blot：各组Bcl-2蛋白相对表达量无显著性意义（P>0.05）;T0组Bax蛋白高于其余各组（P<0.05）,T3组低于T1组（P<0.05）;T0组Bcl-2/Bax值低于N、T3组（P<0.05）,T1、T2组均低于T3组（P<0.05）;T0组Caspase-3蛋白高于其余各组（P<0.05）,N组低于T1组（P<0.05）,T1组高于T3组（P<0.05）。结论：鸢尾素可能通过减少Bax、Caspase-3蛋白表达，升高Bcl-2/Bax值来减轻神经细胞凋亡且与鸢尾素注射剂量相关。更多还原

【Abstract】 Objective:To investigate the effect of irisin on apoptosis of hyperbilirubinemia cells in neonatal rats.Method:Ninety-six newborn SD rats were randomly divided into the control group(N group) and the model group(M group). The model was established by intraperitoneal injection of bilirubin solution at age 7 and 10days. After successful modeling, the M group was randomly divided into four groups: T0, T1, T2, T3.Groups T1,T2,and T3 received intracerebroventricular injections of 40,60,and 80 μg/kg irisin solution,respectively, while the other groups were injected with an equivalent volume of phosphate buffer saline(PBS)solution. After 24 hours, neurobehavioral assessments were conducted,hematoxylin-eosin(HE) staining,terminal-deoxynucleoitidyl transferase mediated Nick end labeling(Tunel) were used to detect the hippocampus pathological changes and apoptosis rate statistics, and Western Blot was used to detect relative expression levels of Bcl-2,Bax and Caspase-3 proteins in hippocampus.Result:After irisin injection, the T3 group required less time needed for planar double positive reflection than the T0,T1,T2 and more than N group(P<0.01);negative geotaxis reflex time was also less in T3 group compared to T0,T1,T2 groups(P<0.01,P<0.05). HE staining showed nuclear pyknosis, cleavage, and disordered arrangement of hippocampal neurons in the M group. The cell morphology was improved to varying degrees in T1,T2 and T3 groups. Tunel staining showed increased Tunel+cells throughout the field in M group,sparse distribution in N group and lower apoptosis rates in T3 group compared to T0 and T1 groups(P<0.05). Western Blot showed no significant difference in the relative expression of Bcl-2 protein among all groups(P>0.05);T0 group had higher Bax protein levels compared to the rest of groups(P<0.05),and lower in T3 group compared to T1 group(P<0.05); The Bcl-2/Bax ratio was lower in T0 group compared to N and T3 group(P<0.05),and lower in T1, T2 groups compared to T3 group(P<0.05);The expression of Caspase-3 protein were higher in T0 group than that in other groups(P<0.05),lower in N group than T1 group(P<0.05),and higher in T1 group than T3 group(P<0.05).Conclusion:Irisin may reduce the apoptosis of nerve cells by reducing the expression of Bax and Caspase-3protein and increasing the Bcl-2/Bax ratio,with effects dependent on the injection dose of irisin.更多还原