【摘要】 目的 了解四川省乙型肝炎(乙肝)病毒(Hepatitis B virus, HBV)基因型和S基因变异。方法 收集2022-2023年四川省11个监测点乙肝病例的乙肝表面抗原阳性血清样本，提取病毒核酸，扩增和测定S基因序列，构建系统进化树，分析HBV基因型和α抗原决定簇氨基酸变异。结果 共获得115株HBV毒株基因序列，其中B基因型50株、C基因型58株和D基因型7株，核苷酸序列同源性分别为94.8%-100%、95.0%-100%和96.0%-99.3%,氨基酸序列同源性分别为92.2%-100%、91.0%-100%和96.0%-99.0%。28株(24.35%)HBV毒株出现α抗原决定簇氨基酸变异，主要分布在该区域的第一环内和126、127、129、131、133、134、140位点；1株在123与124位点之间插入了氨基酸K和A,1株172位点的氨基酸变异为终止密码子，各1株出现新的糖基化位点N129和N131。结论 四川省HBV优势流行株为B和C基因型，且出现一定比例的HBV抗原性改变。更多还原

【Abstract】 Objective To identify genotypes and S gene mutations of hepatitis B virus(HBV) isolates in Sichuan province.Methods We collected hepatitis B surface antigen positive serum samples from hepatitis B cases during 2022-2023 in 11 monitoring sites of Sichuan, extracted HBV nucleic acid, amplified S gene sequences, and constructed a phylogenetic tree. We analyzed HBV genotypes and amino acid mutations in α antigenic determinants.Results We obtained gene sequences of 115 HBV strains, in which 50, 58, and 7 strains were B, C, and D genotype, respectively, with nucleotide homologies of 94.8%-100%, 95.0%-100%, and 96.0%-99.3% and amino acid homologies of 92.2%-100%, 91.0%-100%, and 96.0%-99.0%. Twenty-eight HBV strains(24.35%) had amino acid mutations in α antigenic determinants. Most mutations were in the first ring of α antigenic determinants at sites 126, 127, 129, 131, 133, 134, and 140. One strain had amino acids K and A inserted between sites 123 and 124. One strain had a stop codon amino acid mutation at site 172. New glycosylation sites N129 and N131 were found in another strain.Conclusion The HBV strains circulating in Sichuan were mostly B and C genotype, with some antigenic changes.更多还原